The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is a major pest of pome fruit worldwide. Incorporation of semiochemicals, including the main sex pheromone (codlemone), into codling moth IPM programs has drastically reduced the amount of chemical insecticides needed to control this orchard pest. Odorant receptors located in sensory neuron membranes in the antennae are key sensors in the detection of semiochemicals and trigger downstream signaling events leading to a behavioral response. CpomOR1 is an odorant receptor belonging to the pheromone receptor subfamily in codling moth, and is a prime candidate for being a codlemone receptor based on its high expression levels in male antennae. In this study, the CpomOR1 gene was targeted using CRISPR/Cas9 genome editing to knockdown functional OR1 protein production to determine physiological function(s). By injecting early stage eggs, mutations were successfully introduced, including both deletions and insertions. When attempting to create stable populations of codling moth through mating of males with females containing mutations of the CpomOR1 gene, it was found that fecundity and fertility were affected, with edited females producing nonviable eggs. The role of CpomOR1 in fecundity and fertility in codling moth is unknown and will be the focus of future studies.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1093/jee/tox166 | DOI Listing |
Pest Manag Sci
December 2024
Department of Entomology, Washington State University, Pullman, WA, USA.
Background: Efficacy of insecticides is often determined from apparent yield loses due to a target pest. However, pests can affect yields even when controls work as expected. Further, most pest populations are monitored through adult counts without procedures to assess dynamics of immature stages.
View Article and Find Full Text PDFPest Manag Sci
November 2024
College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Microorganisms
September 2024
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
The use of CpGV strains as the basis for bioinsecticides is an effective and safe way to control . The research is aimed at the identification and study of new CpGV strains. Objects of identification and bioinformatic analysis: 18 CpGV strains.
View Article and Find Full Text PDFPeerJ
September 2024
College of Forestry and Landscape Architecture, Xinjiang Agriculture University, Urumqi, China.
The codling moth () has a major effect on the quality and yield of walnut fruit. Plant defences respond to insect infestation by activating hormonal signalling and the flavonoid biosynthetic pathway. However, little is known about the role of walnut husk hormones and flavonoid biosynthesis in response to infestation.
View Article and Find Full Text PDFInt J Mol Sci
June 2024
Kurchatov Genomic Centre of Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, Russia.
granulovirus is a natural pathogen for that is used as a biocontrol agent of insect populations. The study of granulovirus virulence is of particular interest since the development of resistance in natural populations of has been observed during the long-term use of the Mexican isolate CpGV. In our study, we present the genomes of 18 CpGV strains endemic to southern Russia and from Kazakhstan, as well as a strain included in the commercial preparation "Madex Twin", which were sequenced and analyzed.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!