Objectives: Pentraxin 3 (PTX3) is a multifunctional soluble factor. PTX3 can be involved in the regulation of vasculitis and is expressed in the cytoplasm of neutrophils. As anti-neutrophil cytoplasmic antibody (ANCA) is recognised as a cause of vasculitis, we aimed to discover the role of PTX3 in ANCA production in vivo.
Methods: To this end, we used aluminum salt (alum), which induces neutrophil extracellular traps, as an adjuvant for producing anti-myeloperoxidase-ANCA (MPO-ANCA). Specifically, we intraperitoneally injected alum and recombinant MPO (rMPO) into MPO-deficient mice and then measured the concentration of anti-MPO IgG in their blood. To show the involvement of extracellular PTX3 in this model, we assessed PTX3 protein content and host double-stranded DNA levels in the mice's peritoneal fluid after alum injection. In addition, we simultaneously administered recombinant PTX3, rMPO and alum to MPO-deficient mice to assess the function of PTX3 in producing anti-MPO IgG in vivo.
Results: Anti-MPO IgG was produced by the alum + rMPO immunisation model in MPO-deficient but not wildtype mice. Injection of alum induced extracellular PTX3 as well as double-stranded DNA and dead cells in MPO-deficient mice. Simultaneous injection of recombinant PTX3 with rMPO and alum attenuated the production of anti-MPO IgG in MPO-deficient mice.
Conclusions: Our current findings provide evidence that PTX3 attenuates the production of murine MPO-ANCA.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Bruton's tyrosine kinase is a possible therapeutic target in microscopic polyangiitis.
Arthritis Res Ther
November 2023
Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-Ku, Sapporo, 0600812, Japan.
Article Synopsis
- The study investigates the therapeutic potential of tirabrutinib, a Btk inhibitor, in treating microscopic polyangiitis (MPA), a small-vessel vasculitis linked to pathogenic antibodies.
- Tirabrutinib was administered to rats, showing that it effectively inhibited Btk phosphorylation without reducing the overall number of peripheral B lymphocytes.
- The results indicated that tirabrutinib reduced the formation of neutrophil extracellular traps (NETs) induced by myeloperoxidase and anti-MPO immune complexes, suggesting its potential as a treatment for MPA.
Evaluation of autoantibody profile in healthy subjects after mRNA vaccination against COVID-19.
Int Immunopharmacol
September 2023
Department of Experimental Medicine, University of Tor Vergata, Rome, Italy.
Background: SARS-CoV-2 severe acute respiratory syndrome has rapidly spread worldwide since 2019. All scientific and technological forces have concentrated towards the formulation of vaccines to contain the disease. In less than one year (December 2020) a first messenger RNA vaccine (Comirnaty, BioNTech/Pfizer) was authorized.
View Article and Find Full Text PDFThe onset of autoantibodies in healthcare workers after mRNA based anti-SARS-CoV-2 vaccines: a single centre prospective follow-up study.
Autoimmunity
December 2023
Research and Innovation Department (DAIRI), "SS. Antonio e Biagio e Cesare Arrigo" Hospital, Alessandria, Italy.
Nowadays, data concerning the risk of autoimmune disease after SARS-CoV-2 (COVID-19) vaccination is controversial. The aim of this single centre prospective follow-up study was to evaluate whether healthcare workers (HCWs) vaccinated with BNT162b2 mRNA and mRNA-1273 will show a development and/or a persistence of autoantibodies, focussing on the detection of antibodies against nuclear antigens (antinuclear antibodies, ANA). We enrolled 155 HCWs, however only 108 of them received the third dose and were considered for further analysis.
View Article and Find Full Text PDFAntimyeloperoxidase antibodies modulate inflammatory responses and activate profibrotic pathways in human monocytes.
J Autoimmun
September 2023
School of Immunology and Microbial Sciences, King's College London, UK. Electronic address:
Antimyeloperoxidase (anti-MPO) and antiproteinase 3 (anti-PR3) antibodies are found in anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV). We investigated the effect of both anti-MPO and anti-PR3 IgG on human monocytes. Peripheral blood monocytes were cultured under a range of conditions that included TLR agonists, anti-MPO IgG and anti-PR3 IgG with appropriate controls.
View Article and Find Full Text PDFDetermination of pediatric reference limits for 10 commonly measured autoantibodies.
Clin Chem Lab Med
October 2022
Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, ON, Canada.
Article Synopsis
- The study aimed to create pediatric reference limits for autoimmune disease markers using a healthy cohort of children and adolescents in Canada, enhancing the interpretation of lab results in clinical practice.
- Healthy participants (n=123, aged 1-19) underwent serum autoantibody testing, and reference limits were determined according to established clinical guidelines.
- Results revealed varying proportions of autoantibodies in the samples and established reference limits for 10 markers, which can assist in better lab assessments for pediatric autoimmune conditions globally.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!