Amino acids (AAs) are essential for protein synthesis, neurotransmission and macro molecule biosynthesis. Ala, Gln, Gly, Lys, Val and taurine (Tau) are the most abundant free AAs in mammals, and some of these react with hypochlorite (HOCl/OCl) produced by myeloperoxidase in activated phagocytes to form N-chloroamino acids (NCAA). In this study, we reacted 20 AAs and Tau with sodium hypochlorite (NaOCl), then classified the products into five types (I-V) based on the change in their absorbance. Type I AAs (Ala, Arg, Gln, Gly, Ile, Lys, Phe, Ser, Tau, Thr and Val) generated a typical monochloramine peak at 252 nm, while Type II AAs (Asn and Tyr) and Type III AAs (Glu and Leu) produced peaks at 275 nm and 225 nm, respectively. The Type IV AAs (His, Met and Trp) did not show any distinct absorption peak, and Type V AAs (Asp, Cys and Pro) did not appear to react with NaOCl. The ArgCl and TauCl were stable, while GlnCl, GlyCl, IleCl, LysCl, PheCl and ValCl were less stable and AlaCl, SerCl and ThrCl were the least stable. Tau is the most abundant non-proteinogenic free AA in cellular fluid and has many physiological functions in the nervous, cardiovascular, renal and immune systems. Tau reacts with HOCl to form TauCl, which inhibits the production of proinflammatory mediators such as superoxide, nitric oxide (NO) and interleukins, while increasing the antioxidant proteins in macrophages. We determined the effects of Type I NCAA on cell viability, NO and TNF-α production in LPS-activated RAW 264.7 cells. All Type I NCAA showed dose-dependent cytotoxicity and inhibited LPS-induced NO production. However, only GlnCl, GlyCl, IleCl, LysCl, SerCl and TauCl inhibited LPS-induced TNF-α production. In summary, Type I NCAA showed dose-dependent cytotoxicity and inhibited NO production, while their effects on TNF-α varied. Our results suggest that Type I NCAA may serve as biological regulators similar to TauCl during inflammation.
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