To determine the process parameters of optimal water-extraction and ethanol precipitation method for Xuanbi'antong (XBF) extract, which is a clinically experience formula for coronary disease. Orthogonal test L9(34) was conducted for the study of XBF water-extraction and ethanol precipitation process. Extractum, salvianolic acid B, rhizoma coptidis alkaloid, paeoniflorin, puerarin, ginsenoside Rb₁, ginsenosides and echinacoside were selected as marker components and multi-index comprehensive weighted score was used to select and verify optimal water-extraction and ethanol precipitation process. The optimal extraction process was as follows： XBF was added with 10 times distilled water, decocted for half an hour for 3 times. The best ethanol-precipitation process was established where the ethanol was added up to 70% and precipitated for 24 hours in 1.12 extract density (20 ℃). The optimized water-extraction and ethanol precipitation method is stable and reliable, and can provide reference for further development and utilization of the formula.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4268/cjcmm20160114 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structure Elucidation and Immunostimulatory Activity Evaluation of a Galactoglucan from Hance.
Foods
December 2024
State Key Laboratory for the Modernization of Classical and Famous Prescriptions of Chinese Medicine, No.1899 Meiling Road, Nanchang 330103, China.
Hance has a medicinal history of thousands of years in treating cough, diabetes, and gastrointestinal system diseases, and it is also a medicine food homology (MFH) plant in China. To evaluate the pharmacological activities of polysaccharides from the rhizomes of , polysaccharides were initially obtained by hot-water extraction and the ethanol precipitation method. A homogenous polysaccharide designated as AOP-w was isolated by a DE-52 column.
View Article and Find Full Text PDFMulti-omics analysis reveals the pre-protective mechanism of Dendrobium flexicaule polysaccharide against alcohol-induced gastric mucosal injury.
Int J Biol Macromol
December 2024
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430065, China. Electronic address:
Dendrobium flexicaule (DF) is an endemic plant primarily found in the mountains of central China with important medicinal and edible values. In traditional Chinese medicine, DF has the effects of nourishing stomach and "Yin", and clearing heat. At present, no studies have explored the mechanisms by which Dendrobium flexicaule polysaccharides (DFP) exert pre-protect effects against alcohol-induced gastric mucosal injury.
View Article and Find Full Text PDFModification of a O-acetyl-glucomannan from Dendrobium officinale by selenylation modification and its anti-gastric cancer enhancing activity.
Int J Biol Macromol
December 2024
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China. Electronic address:
In this study, a homogeneous polysaccharide, named YDOP-1 was isolated form Dendrobium officinale using hot water extraction and ethanol precipitation method. YDOP-1 was characterized to be a typical O-acetyl-glucomannan with the molecular wight was 13,456 Da. Cell viability and colony forming assay showed that YDOP-1 possess moderate anti-gastric cancer effects.
View Article and Find Full Text PDFImmunostimulatory effects of rhamnogalacturonan-I fraction purified from Glycyrrhiza glabra roots on cyclophosphamide-induced immunosuppressed mice.
Int J Biol Macromol
December 2024
Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of Korea. Electronic address:
The present study aimed to investigate the immunostimulatory activities of polysaccharides purified from Glycyrrhiza glabra root. First, five polysaccharide fractions were separated from G. glabra through hot water extraction, ethanol precipitation, enzymatic hydrolysis, and size exclusion chromatography.
View Article and Find Full Text PDFStructural Characteristics and Immunological Function of a New Non-Starch Polysaccharide from Taro.
Foods
November 2024
Jiangxi Province Key Laboratory of Vegetable Cultivation and Utilization, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.
Taro is a tuber crop that is used for nutritional and medicinal purposes due to its abundance of non-starch polysaccharides (NSPs). taro is a local variety in Southern China, but the characteristics and bioactivities of its NSPs are currently unknown. In this study, NSPs were isolated from the corms of taro using hot-water extraction, ion-exchange chromatography, and ethanol precipitation; their molecular weight, monosaccharide composition, structural formulae, and immunomodulatory effects were examined.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!