AI Article Synopsis
- Researchers studied the hematopoietic marrow microenvironment and discovered that dermatopontin (DPT), a small protein in the extracellular matrix (ECM), is present and aids in cell adherence.
- * In experiments, DPT was found to work with other ECM proteins to help hematopoietic cells stick to surfaces, but it wasn’t essential for blood cell formation in living organisms.
- * Mice lacking DPT showed no significant differences in bone marrow cell composition or function, indicating that other cell adhesion mechanisms can compensate for its absence.
Article Abstract
The hematopoietic marrow microenvironment is composed of multiple cell types embedded in an extracellular matrix (ECM). We have explored marrow ECM using mass spectrometry and found dermatopontin (DPT), a small non-collagenous ECM protein, to be present. We found that DPT cooperates with other ECM proteins to promote hematopoietic cell adherence in vitro on plastic as well as OP9 stromal cells. We generated constitutional DPT mice that were viable and had no peripheral lympho-hematopoietic abnormalities. The composition of the marrow of wild-type and DPT mice was equivalent in terms of cellularity, CFU-C, LSK (Lineage, SCA-1, KIT), and LSK-SLAM (LSK, CD48, CD150) frequencies. These data suggest that DPT fosters adherence but is not required for steady-state hematopoiesis in vivo. There are likely overlapping cellular adhesion mechanisms that can compensate to maintain the hematopoietic niche in the absence of DPT.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599243 | PMC |
| http://dx.doi.org/10.1016/j.stemcr.2017.07.021 | DOI Listing |
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