Quantification of Embryonic Myofiber Development by Immunofluorescence.

Mammalian myogenesis occurs in two distinct phases, primary and secondary, which are temporally separated. The primary wave occurs shortly after somitogenesis producing embryonic myofibers. The secondary wave occurs after somitogenesis producing fetal myofibers that form adjacent to the embryonic myofibers. The myogenic cells that give rise to these two waves have distinct characteristics as do the myofibers they produce. The objective of this chapter is to describe our methods for quantifying embryonic and fetal myofiber development in mouse embryos using immunofluorescence.