Optimization of Surfactant-Mediated, Ultrasonic-assisted Extraction of Antioxidant Polyphenols from Rattan Tea () Using Response Surface Methodology.

Pharmacogn Mag

Department of Food Science, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212001, Jiangsu Province, P. R. China.

Published: July 2017

AI Article Synopsis

  • Rattan tea is known for its medicinal properties, used historically to treat various health issues like inflammation and diabetes. This study focuses on a new, eco-friendly method to extract beneficial compounds from it.
  • The optimal extraction conditions using Tween-80 as a solvent were found to be 6.8% concentration, 54°C temperature, and 28.8 minutes, leading to high levels of antioxidant activity measured by total phenolic content and ferric-reducing capacity.
  • This surfactant-mediated, ultrasonic-assisted extraction proved to be more efficient, reducing both time and costs compared to traditional extraction methods like heat reflux and ultrasonic extraction with ethanol.

Article Abstract

Background: Rattan tea is a medicinal plant that has been used for many years for the treatment of inflammation, fatty liver, tumor, diabetes, and hyperlipidemia.

Objective: A green and novel approach based on surfactant-mediated, ultrasonic-assisted extraction (SM-UAE) was developed for the extraction of antioxidant polyphenols from Rattan tea. A nonionic surfactant Tween-80 was selected as extraction solvent. The antioxidant activity was measured by total phenolic content (TPC) and ferric-reducing/antioxidant capacity (FRAC) assay.

Materials And Methods: Optimization of extraction parameters including concentration of solvent, ultrasonic time, and temperature were investigated by response surface methodology. The antioxidant activity was measured by TPC and FRAC assay.

Results: The optimal extraction conditions were determined as 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54°C, and ultrasonic time of 28.8 min. Under these conditions, the highest TPC value of 360.4 mg gallic acid equivalent per gram of dry weight material (GAE/g DW) was recorded. Moreover, 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54.5°C, and ultrasonic time of 28.4 min were determined for the highest FRAC value of 478.2 μmol of Fe/g of weight material (μmol Fe/g DW). Compared with other methods, the TPC and FRAC values of 313.5 mg GAE/g DW and 389.6 μmol Fe/g DW were obtained by heat reflux extraction using ethanol as solvent, respectively, and 343.2 mg GAE/g DW and 450.1 μmol Fe/g DW were obtained by UAE using ethanol as solvent, respectively.

Conclusion: The application of SM-UAE markedly decreased extraction time or extraction cost and improved the extraction efficiency, compared with the other methods.

Summary: Surfactant-mediated ultrasonic-assisted extraction of antioxidant polyphenols from Response surface methodology used to optimize parameters and study combined effectsOptimized surfactant-mediated ultrasonic-assisted extraction process enhances the antioxidant phenolics extraction in less time. SM-UAE: Surfactant-mediated ultrasonic-assisted extraction; TPC: total phenolic content; FRAC: Ferric reducing antioxidant capacity; RSM: Response surface methodology; BBD: Box-Behnken design.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551363PMC
http://dx.doi.org/10.4103/pm.pm_159_16DOI Listing

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