Arabidopsis E3 Ubiquitin Ligases PUB22 and PUB23 Negatively Regulate Drought Tolerance by Targeting ABA Receptor PYL9 for Degradation.

Int J Mol Sci

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Published: August 2017

Drought causes osmotic stress and rapidly triggers abscisic acid (ABA) accumulation in plants. The roles of various ABA receptors in drought tolerance and molecular mechanisms regulating ABA receptor stability needs to be elucidated. Here, we report that Arabidopsis plants overexpressing PYL9, one of the 14 pyrabactin resistance (PYR)/pyrabactin resistance-like (PYL)/regulatory component of ABA receptors (RCAR) family ABA receptors, gained drought tolerance trait. Osmotic stress induced accumulation of the PYL9 protein, which was regulated by the 26S proteasome. PYL9 interacted with two highly homologous plant U-box E3 ubiquitin ligases PUB22 and PUB23. In the cell-free degradation assay, the degradation of GST-PYL9 was accelerated in protein extract from plants overexpressing PUB22 but slowed down in protein extract from the double mutant. The in vivo decay of Myc-PYL9 was significantly reduced in the double mutant as compared with the wild-type. Additionally, PUB22 also interacted with other ABA receptors such as PYL5, PYL7 and PYL8. Considering the improved drought tolerance in the double mutant in previous studies, our results suggest that PUB22 and PUB23 negatively regulate drought tolerance in part by facilitating ABA receptors degradation.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618490PMC
http://dx.doi.org/10.3390/ijms18091841DOI Listing

Publication Analysis

Top Keywords

drought tolerance
20
aba receptors
20
pub22 pub23
12
double mutant
12
ubiquitin ligases
8
ligases pub22
8
pub23 negatively
8
negatively regulate
8
regulate drought
8
aba
8

Similar Publications

Background: Drought stress is a significant global challenge that negatively impacts cotton fiber yield and quality. Although many drought-stress responsive genes have been identified in cotton species (Gossypium spp.), the diversity of drought response mechanisms across cotton species remains largely unexplored.

View Article and Find Full Text PDF

Synergistic and antagonistic relationships between cytokinins and other plant growth regulators are important in response to changing environmental conditions. Our study aimed to determine the functions of SlHP2 and SlHP3, two members of cytokinin signaling in tomato, in drought stress response using CRISPR/Cas9-mediated mutagenesis. Ten distinct genome-edited lines were generated via Agrobacterium tumefaciens-mediated gene transfer and confirmed through Sanger sequencing.

View Article and Find Full Text PDF

Two pepper subclass II SnRK2 genes positively regulate drought stress response, with differential responsiveness to abscisic acid.

Plant Physiol Biochem

January 2025

Department of Life Science (BK21 Program), Chung-Ang University, 84 Heukseok-Ro, Dongjak-Gu, 06974, Seoul, Republic of Korea. Electronic address:

Sucrose nonfermenting-1-related protein kinase 2 (SnRK2) intricately modulates plant responses to abiotic stresses and abscisic acid (ABA) signaling. In pepper genome, five SnRK2 genes with sequence homology to CaSnRK2.6 showed distinct expression patterns across various pepper organs and in response to treatments with ABA, drought, mannitol, and salt.

View Article and Find Full Text PDF

Introduction: The increase in vapor pressure deficit (VPD) is among the expected change in futur climate, and understanding its effect on crop growth is of much significance for breeeding programs. Three groups (G1,G2 and G3) of pearl millet germplasm, originating from regions with different rainfall intensities, were grown in the field during period of high and low VPDs. The groups G1,G2 and G3 were respectively from Guinean (rainfall above 1000 mm), Soudanian (rainfall between 600 mm and 900 mm), and Sahelian zones (rainfall between 600 and 300 mm) of Africa.

View Article and Find Full Text PDF

Leveraging RNA interference technology for selective and sustainable crop protection.

Front Plant Sci

December 2024

State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, China.

Double-stranded RNA (dsRNA) has emerged as key player in gene silencing for the past two decades. Tailor-made dsRNA is now recognized a versatile raw material, suitable for a wide range of applications in biopesticide formulations, including insect control to pesticide resistance management. The mechanism of RNA interference (RNAi) acts at the messenger RNA (mRNA) level, utilizing a sequence-dependent approach that makes it unique in term of effectiveness and specificity compared to conventional agrochemicals.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!