AI Article Synopsis
- L. is being studied for its benefits in nutrition and medicine, with a focus on boosting its antioxidant properties through increased flavonoid and phenolic content.
- The research involved transforming hairy root cultures with a specific strain, confirming genetic changes, and measuring gene expression levels related to flavonoid production.
- Results indicated significant increases in phenolic and flavonoid content as well as antioxidant potential in the transgenic roots compared to nontransgenic roots, suggesting a successful enhancement of beneficial compounds.
Article Abstract
L. is a herbaceous species, used for human nutrition and medicinal purposes. The high antioxidant capacity of indicates the possibility of enhancing its edible and health potential by increasing the flavonoid and phenolic contents. The present study aimed at enhancing the production of phenolics and flavonoids by hairy root cultures in transformed with strain AR15834 harbouring the gene. The genetic transformation of in transformed roots was validated, and expression level was evaluated using real-time qPCR analysis. Expression levels of flavonoid biosynthesis genes (CHI, PAL, FLS, and CHS) were assessed in the hairy and nontransformed roots. Results showed higher expression levels in the transgenic roots than in the nontransformed ones ( < 0.01). Transgenic hairy roots exhibited a 54.8-96.7% increase in the total phenolic content, 38.1-76.2% increase in the total flavonoid content, and 56.7-96.7% increase in the total reducing power when compared with the nontransgenic roots ( < 0.01). DPPH results also revealed that the transgenic hairy roots exhibited a 31.6-50% increase in antioxidant potential, when compared to normal roots. This study addressed the enhancement of secondary metabolite biosynthesis by hairy root induction in .
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5556996 | PMC |
| http://dx.doi.org/10.1155/2017/5604746 | DOI Listing |
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