Neutrophils play important roles in innate immunity and are mainly dependent on various enzyme-containing granules to kill engulfed microorganisms. Zebrafish () is specifically expressed in neutrophils; however, its function is largely unknown. Here, we generated an mutant ( ) via CRISPR/Cas9 to investigate the function of Npsn. The overall development and number of neutrophils remained unchanged in -deficient mutants, whereas neutrophil antibacterial function was defective. Upon infection with , the mutants exhibited a lower survival rate and more severe bacterial burden, as well as augmented inflammatory response to challenge with infection when compared with wild-type embryos, whereas -overexpressing zebrafish exhibited enhanced host defence against infection. These findings demonstrated that zebrafish Npsn promotes host defence against bacterial infection. Furthermore, our findings suggested that -deficient and -overexpressing zebrafish might serve as effective models of innate immunity.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577445 | PMC |
| http://dx.doi.org/10.1098/rsob.170040 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Understanding phage BX-1 resistance in AP-1 and the role of quorum-sensing regulation.
Microbiol Spectr
January 2025
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
The marine ecosystem is characterized by a rich diversity of bacterial hosts and their phages. The propagation of phages is primarily limited by their ability to adsorb to host cells and is further challenged by various bacterial defense mechanisms. To fully realize the potential of phage therapy in aquaculture, a comprehensive understanding of phage-host interactions and their regulation is essential.
View Article and Find Full Text PDFAntiviral defense systems in the rumen microbiome.
mSystems
January 2025
Institute of Animal Science, University of Hohenheim, Stuttgart, Germany.
The continuous interaction between phages and their respective hosts has resulted in the evolution of multiple bacterial immune mechanisms. However, the diversity and prevalence of antiviral defense systems in complex communities are still unknown. We therefore investigated the diversity and abundance of viral defense systems in 3,038 high-quality bacterial and archaeal genomes from the rumen.
View Article and Find Full Text PDFTomato Defenses Under Stress: The Impact of Salinity on Direct Defenses Against Insect Herbivores.
Plant Cell Environ
January 2025
Department of Entomology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Abiotic stressors, such as salt stress, can reduce crop productivity, and when combined with biotic pressures, such as insect herbivory, can exacerbate yield losses. However, salinity-induced changes to plant quality and defenses can in turn affect insect herbivores feeding on plants. This study investigates how salinity stress in tomato plants (Solanum Lycopersicum cv.
View Article and Find Full Text PDFMitochondrial dysfunction is a major cause of thromboinflammation and inflammatory cell death in critical illnesses.
Inflamm Res
January 2025
Department of Anesthesiology, Critical Care, and Surgery, Duke University School of Medicine, Durham, NC, USA.
Background: Mitochondria generate the adenosine triphosphate (ATP) necessary for eukaryotic cells, serving as their primary energy suppliers, and contribute to host defense by producing reactive oxygen species. In many critical illnesses, including sepsis, major trauma, and heatstroke, the vicious cycle between activated coagulation and inflammation results in tissue hypoxia-induced mitochondrial dysfunction, and impaired mitochondrial function contributes to thromboinflammation and cell death.
Methods: A computer-based online search was performed using the PubMed and Web of Science databases for published articles concerning sepsis, trauma, critical illnesses, cell death, mitochondria, inflammation, coagulopathy, and organ dysfunction.
Shallow-water mussels (Mytilus galloprovincialis) adapt to deep-sea environment through transcriptomic and metagenomic insights.
Commun Biol
January 2025
University of Chinese Academy of Sciences, 10049, Beijing, China.
Recent studies have unveiled the deep sea as a rich biosphere, populated by species descended from shallow-water ancestors post-mass extinctions. Research on genomic evolution and microbial symbiosis has shed light on how these species thrive in extreme deep-sea conditions. However, early adaptation stages, particularly the roles of conserved genes and symbiotic microbes, remain inadequately understood.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!