An enzymatic strategy was developed to generate asymmetrically branched N-glycans from natural sources by using a panel of glycosidases and glycosyltransferases. Briefly, LacZ β-galactosidase was employed to selectively trim symmetrically branched N-glycans isolated from bovine fetuin. The yielding structures were then converted to asymmetrically branched core structures by robust glycosyltransferase for further extension.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603075 | PMC |
http://dx.doi.org/10.1039/c7ob01765k | DOI Listing |
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