An enzymatic strategy to asymmetrically branched N-glycans.

Org Biomol Chem

Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA.

Published: September 2017

An enzymatic strategy was developed to generate asymmetrically branched N-glycans from natural sources by using a panel of glycosidases and glycosyltransferases. Briefly, LacZ β-galactosidase was employed to selectively trim symmetrically branched N-glycans isolated from bovine fetuin. The yielding structures were then converted to asymmetrically branched core structures by robust glycosyltransferase for further extension.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603075PMC
http://dx.doi.org/10.1039/c7ob01765kDOI Listing

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