Cuticular surface damage of adult worms treated with betel nuts extract .

Aim: The objective of this research was to evaluate the cuticular surface damage of adult worms treated with ethanolic extract of betel nuts .

Materials And Methods: Phytochemical screening was done using FeCl, Wagner and Dragendorff reagents, NaOH, MgHCl, and Liebermann-Burchard reaction test. Amount of 16 worms were segregated into four groups with three replicates. Four worms of each group submerged into phosphate buffered saline, 25 mg/ml, and 75 mg/ml crude ethanolic extract of , and 15 mg/ml albendazole. The effect of these extract was observed 40 h after incubation as soon as worms death. The worms were sectioned transversally and were explored for any cuticular histopathological changes in their body surface under microscope.

Results: We found that the ethanolic extract of betel nuts contains tannins, alkaloids, flavonoids, triterpenoids, and saponins. The ethanolic extract of betel nuts induces surface alterations caused cuticular damage of adult worms.

Conclusion: We concluded that ethanolic extract of betel nuts possess anthelmintic activity caused cuticular damage of adult worms.