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http://dx.doi.org/10.1007/s00401-017-1764-0 | DOI Listing |
J Biol Chem
December 2024
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, Japan. Electronic address:
The mechanistic target of rapamycin complex 1 (mTORC1) functions as a master regulator of cell growth and proliferation. We previously demonstrated that intracellular calcium ion (Ca) concentration modulates the mTORC1 pathway via binding of the Ca sensor protein calmodulin (CaM) to tuberous sclerosis complex 2 (TSC2), a critical negative regulator of mTORC1. However, the precise molecular mechanism by which Ca/CaM modulates mTORC1 activity remains unclear.
View Article and Find Full Text PDFNat Prod Res
December 2024
Phytochemistry Department, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C, Tehran, Iran.
Plant-based nano-insecticides like provide eco-friendly pest control with low resistance risk. This study aimed to evaluate the insecticidal activity of the FeO @Carbon nanoformulation of extract with a carbon shell and pure extract against (eggs and larvae), a significant potato pest in Iran. A modified solvothermal method produced highly water-dispersible magnetite (FeO) particles, with citrate as a stabilising agent.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
The ubiquitin-proteasome system (UPS) is a key protein degradation pathway in eukaryotes, in which E3 ubiquitin ligases mediate protein ubiquitination, directly or indirectly targeting substrate proteins to regulate various biological processes, including plant growth, hormone signaling, immune responses, and adaptation to abiotic stress. In this study, we identified plant U-box protein 51 in () as an E3 ubiquitin ligase through transcriptomic analysis, and used it as a candidate gene for gene-function analysis. Quantitative real-time PCR (qRT-PCR) was used to examine expression across different tissues, and its expression patterns under simulated drought stress induced by polyethylene glycol (PEG 6000) were assessed.
View Article and Find Full Text PDFFood Chem
December 2024
Anhui Province Key Laboratory of Non-coding RNA Basic and Clinical Transformation (Wannan Medical College), Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui, China; Central Laboratory, Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui, China. Electronic address:
Bletilla striata polysaccharide (BSP) has attracted considerable interest due to its diverse biological activities. In this research, a novel low-molecular-weight water-soluble polysaccharide (BSP-182) was isolated and purified from Bletilla striata tubers, and its structure was characterized. The findings indicated that BSP-182 is predominantly composed of β-1,4-linked glucose (Glc) and β-1,4-linked mannose (Man) in a molar ratio of approximately 7.
View Article and Find Full Text PDFCRISPR J
December 2024
Section for Plant Glycobiology, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg C, Denmark.
The potato family includes a highly diverse cultivar repertoire and has a high potential for nutritional yield improvement and refinement but must in line with other crops be adapted to biotic and abiotic stresses, for example, accelerated by climate change and environmental demands. The combination of pluripotency, high ploidy, and relative ease of protoplast isolation, transformation, and regeneration together with clonal propagation through tubers makes potato highly suitable for precise genetic engineering. Most potato varieties are tetraploid having a very high prevalence of length polymorphisms and small nucleotide polymorphisms between alleles, often complicating CRISPR-Cas editing designs and strategies.
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