Methylene blue as a lignin surrogate in manganese peroxidase reaction systems.

Anal Biochem

School of Chemical, Biological, and Environmental Engineering, 116 Johnson Hall, Oregon State University, Corvallis, OR 97331, USA. Electronic address:

Published: November 2017

Manganese peroxidase (MnP) is associated with lignin degradation and is thus relevant to lignocellulosic-utilization technologies. Technological applications require reaction mixture optimization. A surrogate substrate can facilitate this if its susceptibility to degradation is easily monitored and mirrors that of lignin. The dye methylene blue (MB) was evaluated in these respects as a surrogate substrate by testing its reactivity in reaction mixtures containing relevant redox mediators (dicarboxylic acids, fatty acids). Relative rates of MB degradation were compared to available literature reports of lignin degradation under similar conditions, and suggest that MB can be a useful lignin surrogate in MnP systems.

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http://dx.doi.org/10.1016/j.ab.2017.08.010DOI Listing

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