AI Article Synopsis
- Enumerating circulating tumor cells (CTCs) and disseminated tumor cells (DTCs) is important for predicting clinical outcomes but is technically challenging due to their rarity.
- A new detection method has been developed that combines EPCAM-based immunomagnetic enrichment with flow cytometry (IE/FC) for accurate quantification.
- This two-step assay takes about 2 hours and allows for the isolation of pure tumor cells for further molecular analysis.
Article Abstract
Enumerating circulating tumor cells (CTCs) in blood and disseminated tumor cells (DTCs) in bone marrow has shown to be clinically useful, as elevated numbers of these cells predict poor clinical outcomes. Accurate detection and quantification is, however, difficult and technically challenging because CTCs and DTCs are extremely rare. We have developed a novel quantitative detection method for enumeration of CTCs and DTCs. Our approach consists of two steps: (1) EPCAM-based immunomagnetic enrichment followed by (2) flow cytometry (IE/FC). The assay takes approximately 2 h to complete. In addition to tumor cell enumeration, IE/FC offers opportunities for direct isolation of highly pure tumor cells for downstream molecular characterization.
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| http://dx.doi.org/10.1007/978-1-4939-7144-2_17 | DOI Listing |
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