In our recent paper, we biochemically analyzed autophagosome-related membranes at the initiation stage of macroautophagy/autophagy using atg knockout (KO) cells and demonstrated that the ULK complex is recruited to 2 distinct membranes: the ER membrane and ATG9A-positive autophagosome precursors. We have also identified phosphatidylinositol synthase (PIS)-enriched ER subdomains as the initiation site of autophagosome formation. Based on these findings, we propose that the ULK complex, the PIS-enriched ER subdomain, and ATG9A vesicles together initiate autophagosome formation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640204 | PMC |
| http://dx.doi.org/10.1080/15548627.2017.1358344 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of new tissue markers for the monitoring and standardization of penile cancer according to the degree of differentiation.
Histol Histopathol
November 2024
Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, Network Biomedical Research Center for Liver and Digestive Diseases (CIBEREHD), University of Alcalá, Alcala de Henares, Spain.
Article Synopsis
- - Penile cancer is a rare type of tumor, more frequently seen in low- and middle-income countries, with major risk factors being age, ethnicity, smoking, hygiene, and HPV infection.
- - Early detection can lead to high cure rates (up to 80%), but late diagnosis significantly lowers survival chances, especially when the cancer has spread.
- - The study assesses various biomarkers related to cell growth, inflammation, and other processes in penile squamous cell carcinoma, suggesting that the degree of cell differentiation affects these biomarkers' expression, which may help in predicting disease outcomes.
ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling.
Pol J Pathol
October 2024
Department of Ophthalmic Center, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
Retinoblastoma is the most common primary intraocular malignancy of childhood. The aim of our study was to investigate the role and regulatory mechanism of the long non-coding RNA ANRIL in retinoblastoma. Here, our data demonstrated that ANRIL overexpression inhibited miR-328-3p expression, but promoted expression of autophagy-related proteins (LC3B, ATG5, and BECN1).
View Article and Find Full Text PDFEmerging roles of ATG9/ATG9A in autophagy: implications for cell and neurobiology.
Autophagy
November 2024
Department of Medical and Biological Sciences, The Catholic University of Korea, Bucheon, South Korea.
Atg9, the only transmembrane protein among many autophagy-related proteins, was first identified in the year 2000 in yeast. Two homologs of Atg9, ATG9A and ATG9B, have been found in mammals. While ATG9B shows a tissue-specific expression pattern, such as in the placenta and pituitary gland, ATG9A is ubiquitously expressed.
View Article and Find Full Text PDFCell-specific Nav1.6 knockdown reduced astrocyte-derived Aβ by reverse Na-Ca transporter-mediated autophagy in alzheimer-like mice.
J Adv Res
July 2024
Department of Physiology, College of Basic Medical Sciences, Liaoning Provincial Key Laboratory of Cerebral Diseases, National-Local Joint Engineering Research Center for Drug-Research and Development (R&D) of Neurodegenerative Diseases, Dalian Medical University, Dalian, Liaoning 116044, China. Electronic address:
Introduction: Nav1.6 is closely related to the pathology of Alzheimer's Disease (AD), and astrocytes have recently been identified as a significant source of β-amyloid (Aβ). However, little is known about the connection between Nav1.
View Article and Find Full Text PDFPho81 is a novel regulator of pexophagy induced by phosphate starvation.
Autophagy
July 2024
Life Sciences Institute and Department of Molecular, Cellular and Development Biology, University of Michigan, Ann Arbor, MI, USA.
In the budding yeast , macroautophagy/autophagy can be induced by various types of starvation. It is thought that potential autophagic substrates vary to meet specific nutritional demands under different starvation conditions. In a recent study, Gross .
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!