AI Article Synopsis
- The OSKM genes (Oct4, Sox2, Klf4, and c-Myc) can reprogram somatic cells into induced pluripotent stem (iPS) cells, a process well established in mammals.
- This study focuses on developing protocols for creating and working with iPS cells in chickens, an avian model.
- The protocols cover the establishment, characterization, maintenance, differentiation, and injection of potential reprogrammed chicken iPS cells.
Article Abstract
In mammals, the introduction of the OSKM (Oct4, Sox2, Klf4, and c-Myc) genes into somatic cells has allowed generating induced pluripotent stem (iPS) cells. So far, this process has been only clearly demonstrated in mammals. Here, using chicken as an avian model, we describe a set of protocols allowing the establishment, characterization, maintenance, differentiation, and injection of putative reprogrammed chicken Induced Pluripotent Stem (iPS) cells.
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| http://dx.doi.org/10.1007/978-1-4939-7216-6_14 | DOI Listing |
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