AI Article Synopsis
- - The study evaluated the performance of the Bioneer AccuPower® HIV-1 Quantitative RT-PCR kit by comparing it to the established Roche COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 test using 288 plasma samples.
- - Results showed a strong correlation between the two assays, with high agreement (94.9%) and a mean difference of only 0.11 Log10 IU/mL, indicating reliability in measurements.
- - The findings suggest that the Bioneer assay is a viable option for quantifying HIV-1 RNA in clinical settings, supporting its use in therapeutic strategy development.
Article Abstract
Background: Measurement of viral load in human immunodeficiency virus type 1 (HIV-1) infected patients is essential for the establishment of a therapeutic strategy. Several assays based on qPCR are available for the measurement of viral load; they differ in sample volume, technology applied, target gene, sensitivity and dynamic range. The Bioneer AccuPower® HIV-1 Quantitative RT-PCR is a novel commercial kit that has not been evaluated for its performance.
Objective: This study aimed to evaluate the performance of the Bioneer AccuPower® HIV-1 Quantitative RT-PCR kit.
Methods: In total, 288 EDTA plasma samples from the Dharmais Cancer Hospital were analyzed with the Bioneer AccuPower® HIV-1 Quantitative RT-PCR kit and the Roche COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 version 2.0 (CAP/CTM v2.0). The performance of the Bioneer assay was then evaluated against the Roche CAP/CTM v2.0.
Results: Overall, there was good agreement between the two assays. The Bioneer assay showed significant linear correlation with CAP/CTM v2.0 (R2=0.963, p<0.001) for all samples (N=118) which were quantified by both assays, with high agreement (94.9%, 112/118) according to the Bland-Altman model. The mean difference between the quantitative values measured by Bioneer assay and CAP/CTM v2.0 was 0.11 Log10 IU/mL (SD=0.26).
Conclusion: Based on these results, the Bioneer assay can be used to quantify HIV-1 RNA in clinical laboratories.
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| http://dx.doi.org/10.12932/AP0865 | DOI Listing |
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