Erylosides F, V-V, and W-W - New triterpene oligoglycosides from the Carribean sponge Erylus goffrilleri.

Seven new triterpene glycosides, erylosides F (1), V (2), V (3), V (4), W (5), W (6) and W (7), were isolated from a Carribean sponge Erylus goffrilleri collected from the Carribean Sea near the Arrecife-Seco reef (Cuba). Structures of the glycosides were determined using H and C NMR spectroscopy, including 2D NMR procedures (H-H COSY, H-С HMBC, H-С HSQC and NOESY) and HR ESI mass spectrometry. Erylosides 1 and 4 have 14-carboxy-24,25-dimethyllanost-8(9)-en-3β,25-diol as aglycone whereas glycosides 2, 3, 5, 6 and 7 are 14-carboxy-24-acetoxy-24-methyllanost-8(9)-en-3β-ol glycoconjugates. Cytotoxic activities of the isolated compounds against Ehrlich carcinoma cells and hemolysis were examined and their dependence on eryloside structure was evaluated.