AI Article Synopsis
- A pandemic of pseudorabies virus (PRV) variant strains is currently affecting China, with limited research conducted on the genes related to PRV pathogenicity.
- The researchers used CRISPR/Cas9 technology to knock out ten potential virulence genes and tested their effects on pathogenicity in a mouse model.
- Findings revealed that the thymidine kinase (TK) and glycoprotein M (gM) genes were linked to reduced virulence, while other genes did not show significant differences, providing insights for future vaccine development.
Article Abstract
There is currently a pandemic of pseudorabies virus (PRV) variant strains in China. Despite extensive research on PRV variant strains in the past two years, few studies have investigated PRV pathogenicity-related genes. To determine which gene(s) is/are linked to PRV virulence, ten putative virulence genes were knocked out using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 technology. The pathogenicity of these mutants was evaluated in a mouse model. Our results demonstrated that of the ten tested genes, the thymidine kinase (TK) and glycoprotein M (gM) knockout mutants displayed significantly reduced virulence. However, mutants of other putative virulence genes, such as glycoprotein E (gE), glycoprotein I (gI), Us2, Us9, Us3, glycoprotein G (gG), glycoprotein N (gN) and early protein 0 (EP0), did not exhibit significantly reduced virulence compared to that of the wild-type PRV. To our knowledge, this study is the first to compare virulence genes from the current pandemic PRV variant strain. This study will provide a valuable reference for scientists to design effective live attenuated vaccines in the future.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552686 | PMC |
| http://dx.doi.org/10.1038/s41598-017-08269-3 | DOI Listing |
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Article Synopsis
- Pseudorabies (PR) is an infectious disease caused by the pseudorabies virus (PRV), and this study focused on detecting and analyzing the recent outbreaks of genotype II PRV.
- Researchers isolated brain tissues suspected of PRV infection, sequenced the virulence-related genes, and conducted pathogenicity evaluations using mice and piglets.
- The isolated strain, named PRV TJbd2023, showed high pathogenicity, causing severe symptoms and a 66.7% mortality rate in suckling piglets, indicating it may be linked to recent outbreaks and vaccine failures in Tianjin, China.
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