AI Article Synopsis
- Vesosomes, a type of self-assembling structure, offer advantages over traditional liposomes due to their stability and ability to encapsulate materials hierarchically.
- Researchers introduced "glucosomes," sugar-based vesicles-in-vesicles made from a natural microbial glycolipid sourced from yeast.
- These glucosomes formed spontaneously in water within a specific pH range (6 to 4) at room temperature, and their formation mechanism was studied using advanced techniques like small angle X-ray scattering and cryo-transmission electron microscopy.
Article Abstract
Vesicle-in-vesicle self-assembled containers, or vesosomes, are promising alternatives to liposomes because of their possible hierarchical encapsulation and high stability. We report herein the first example of sugar-based vesicles-in-vesicles, which we baptize glucosomes. These were prepared by using a natural microbial glycolipid (branched C22 sophorolipid) extracted from the culture medium of the yeast . Glucosomes spontaneously formed in water between pH 6 and pH 4 at room temperature, without the requirement of any additive. By means of pH-resolved in situ small angle X-ray scattering, we provided direct evidence for the vesicle-formation mechanism. Statistical treatment of the vesicle radii distribution measured by cryo-tansmission electron microscopy by using a derived form of the Helfrich bending free-energy expression provided an order of magnitude for the effective bending constant (the sum of the curvature and the saddle-splay moduli) of the lipid membrane to (0.4±0.1) . This value is in agreement with the bending constant measured for hydrocarbon-based vesicles membranes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542763 | PMC |
| http://dx.doi.org/10.1002/open.201700101 | DOI Listing |
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