The Histone Deacetylase Inhibitor JAHA Down-Regulates pERK and Global DNA Methylation in MDA-MB231 Breast Cancer Cells.

Materials (Basel)

Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Edificio 16, Università di Palermo, Viale delle Scienze, Palermo 90128, Italy.

Published: October 2015

The histone deacetylase inhibitor N¹-(ferrocenyl)-N⁸-hydroxyoctanediamide (JAHA) down-regulates extracellular-signal-regulated kinase (ERK) and its activated form in triple-negative MDA-MB231 breast cancer cells after 18 h and up to 30 h of treatment, and to a lesser extent AKT and phospho-AKT after 30 h and up to 48 h of treatment. Also, DNA methyltransferase 1 (DNMT1), 3b and, to a lesser extent, 3a, downstream ERK targets, were down-regulated already at 18 h with an increase up to 48 h of exposure. Methylation-sensitive restriction arbitrarily-primed (MeSAP) polymerase chain reaction (PCR) analysis confirmed the ability of JAHA to induce genome-wide DNA hypomethylation at 48 h of exposure. Collective data suggest that JAHA, by down-regulating phospho-ERK, impairs DNMT1 and 3b expression and ultimately DNA methylation extent, which may be related to its cytotoxic effect on this cancer cytotype.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5455366PMC
http://dx.doi.org/10.3390/ma8105358DOI Listing

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