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Premise Of The Study: Sixteen microsatellite markers were developed to study the fine-scale spatial genetic structure of , a dioecious tree species of Theaceae endemic to southern China.
Methods And Results: A total of 30 primer pairs were initially designed and tested on the basis of the transcriptome data of , of which 16 were successfully amplified and showed clear polymorphism. For these microsatellites, one to 17 alleles per locus were identified. The observed and expected heterozygosities ranged from 0 to 1.000 and 0 to 0.903, respectively.
Conclusions: The microsatellite markers described here can be used to study genetic diversity and spatial genetic structure of . Furthermore, all loci were successfully cross-amplified in a related species, .
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http://dx.doi.org/10.3732/apps.1700037 | DOI Listing |
Discov Oncol
March 2025
Department of Laboratory Medicine, Shenzhen Guangming District People's Hospital, Shenzhen, 518000, Guangdong, China.
Purpose: To investigate the expression and clinical significance of insulin-like growth factor 2 mRNA-binding protein family members (IGF2BPs) in pan-cancer and evaluate their potential as targets for tumor immunotherapy.
Methods: Based on data from the cancer genome atlas (TCGA) database, pan-cancer analysis was conducted to examine the clinical significance of IGF2BPs expression in twenty-two tumors.
Results: Differential expression analysis showed high expression of IGF2BPs in most tumor tissues.
Mol Ecol
March 2025
Program in Public Health, University of California, Irvine, California, USA.
Anti-malaria interventions typically reduce the intensity of Plasmodium transmission, but the effects of reduced transmission on P. falciparum population biology remain unclear. Highly polymorphic microsatellite markers in P.
View Article and Find Full Text PDFSci Rep
March 2025
Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Gödöllő - Keszthely, 2100, Hungary.
The crucian carp (Carassius carassius Linnaeus, 1758) is a declining native European cyprinid, inhabiting small water bodies, primariliy threatened by climate change, anthropogenic impacts and invasive relative the Prussian carp. Despite conservation efforts across Europe, data on Carphatian Basin populations remain scarce. This study analyzed nine natural populations (257 individuals) in Hungary using thirteen microsatellite markers and mitochondrial DNA COI sequencing (187 individuals).
View Article and Find Full Text PDFBMC Plant Biol
March 2025
Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Southwest University, Chongqing, 400715, China.
Sweet potato (Ipomoea batatas (L.) Lam.) is a vital global crop, with breeding focused on both high starch and high yield.
View Article and Find Full Text PDFSci Rep
March 2025
Department of Pathology, College of Basic Medicine, Chongqing Medical University, Chongqing, China.
To elucidate the relationship between circulating cytokines and the prognosis of microsatellite-stable(MSS) colorectal cancer (CRC) patients, we examined the correlation between circulating cytokine levels and tumor immune infiltration microenvironment in this patient population. By conducting a preliminary analysis of the GEO database, we identified five core genes associated with colorectal cancer and further analyzed their impact on immune infiltration. We measured serum cytokine levels and validated the immune infiltration results through immunohistochemical staining of common inflammatory cell markers, including CD3, CD4, CD8, CD163, and FOXP3.
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