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Development of microsatellite markers for (Theaceae). | LitMetric

Development of microsatellite markers for (Theaceae).

Appl Plant Sci

State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.

Published: July 2017

Premise Of The Study: Sixteen microsatellite markers were developed to study the fine-scale spatial genetic structure of , a dioecious tree species of Theaceae endemic to southern China.

Methods And Results: A total of 30 primer pairs were initially designed and tested on the basis of the transcriptome data of , of which 16 were successfully amplified and showed clear polymorphism. For these microsatellites, one to 17 alleles per locus were identified. The observed and expected heterozygosities ranged from 0 to 1.000 and 0 to 0.903, respectively.

Conclusions: The microsatellite markers described here can be used to study genetic diversity and spatial genetic structure of . Furthermore, all loci were successfully cross-amplified in a related species, .

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5546169PMC
http://dx.doi.org/10.3732/apps.1700037DOI Listing

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