Amplification and Mutations in Glioblastoma Patients of the Northeast of Morocco.

Glioblastomas are the most frequent and aggressive primary brain tumors which are expressing various evolutions, aggressiveness, and prognosis. Thus, the 2007 World Health Organization classification based solely on the histological criteria is no longer sufficient. It should be complemented by molecular analysis for a true histomolecular classification. The new 2016 WHO classification of tumors of the central nervous system uses molecular parameters in addition to histology to reclassify these tumors and reduce the interobserver variability. The aim of this study is to determine the prevalence of mutations and amplifications in the population of the northeast region of Morocco and then to compare the results with other studies. . codon 132 and codon 172 were directly sequenced and the amplification of exon 20 of gene was investigated by qPCR in 65 glioblastoma tumors diagnosed at the University Hospital of Fez between 2010 and 2014. . The R132H mutation was observed in 8 of 65 tumor samples (12.31%). No mutation of was detected. amplification was identified in 17 cases (26.15%). . A systematic search of both histological and molecular markers should be requisite for a good diagnosis and a better management of glioblastomas.