Insects rely on their sense of smell to guide a wide range of behaviors that are critical for their survival, such as food-seeking, predator avoidance, oviposition, and mating. Myriad chemicals of varying volatilities have been identified as natural odorants that activate insect Olfactory Receptor Neurons (ORNs). However, studying the olfactory responses to low-volatility odorants has been hampered by an inability to effectively present such stimuli using conventional odor-delivery methods. Here, we describe a procedure that permits the effective presentation of low-volatility odorants for in vivo Single-Sensillum Recording (SSR). By minimizing the distance between the odor source and the target tissue, this method allows for the application of biologically salient but hitherto inaccessible odorants, including palmitoleic acid, a stimulatory pheromone with a demonstrated effect on ORNs involved in courtship and mating behavior. Our procedure thus affords a new avenue to assay a host of low-volatility odorants for the study of insect olfaction and pheromone communication.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613777 | PMC |
| http://dx.doi.org/10.3791/56147 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Aerosol Alteration of Behavioral Response to Pheromone in Bombyx mori.
J Chem Ecol
August 2023
Insect Biology Research Institute, University of Tours - CNRS, Tours, France.
Article Synopsis
- Aerosols have been largely overlooked in olfaction studies, despite their prevalence in the atmosphere and ability to interact with odor molecules like pheromones.
- A study focused on male moths (Bombyx mori) showed that their arousal behavior was affected by aerosol concentrations when exposed to bombykol, the primary component of their sex pheromone.
- The research indicates that aerosols consistently interact with pheromones, with lower aerosol concentrations leading to better moth responses, and proposes hypotheses about competition for olfactory receptors and potential positive effects of aerosols based on their physical properties.
Individual adsorption of low volatility pheromones: Amphiphilic molecules on a clean water-air interface.
J Chem Phys
September 2022
ICSM, CEA, CNRS, ENSCM, Univ. Montpellier, Marcoule, France.
Environmental conditions can alter olfactory scent and chemical communication among biological species. In particular, odorant molecules interact with aerosols. Thermodynamics variables governing the adsorption from air to water surface of bombykol, the most studied pheromone, and of three derivative molecules, bombykal, bombykoic acid, and bombykyle acetate, are computed by steered and un-biased molecular dynamics in order to compare the role of their polar head group on adsorption on aqueous aerosols.
View Article and Find Full Text PDFImmobilization of phosphonium-based ionic liquid stationary phases extends their operative range to routine applications in the flavor, fragrance and natural product fields.
J Chromatogr A
February 2022
Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, I-10125 Turin, Italy.
Phosphonium-based ionic liquids (ILs) have proven to be successful stationary phases (SPs) for gas chromatography (GC) in several fields of application because of their unique selectivity and good chromatographic properties. This study focuses on the use of two ILs as GC SPs that are based on the phosphonium derivatives trihexyl(tetradecyl)phosphonium chloride ([P] [Cl]), and trihexyl(tetradecyl)phosphonium bis[(trifluoromethyl)sulfonyl]imide ([P][NTf]), which have previously been shown to be complementary in terms of chromatographic selectivity and retention. Their application in routine analysis has been limited by their lower maximum allowable operating temperatures (MAOT) (200 °C for the [P][Cl] IL and 180 °C for [P][NTf]), which restricts their use to samples that consist of analytes with relatively high volatility.
View Article and Find Full Text PDFTwo-temperature preparation method for PDMS-based canine training aids for explosives.
Forensic Chem
December 2020
Canine Performance Sciences Program, College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
Canine training aids based on vapor capture-and-release into a flexible polymer, polydimethylsiloxane (PDMS), have been used for in canine detection of explosives that have volatile or semi-volatile odorants. To enhance the rate of odor capture for less volatile targets, two temperatures are used for aid preparation. By using an elevated temperature for the target explosive, the amount of vapor is enhanced, increasing the production of the characteristic odor profile.
View Article and Find Full Text PDFRapid Analysis of Volatile Phenols from Grape Juice by Immersive Sorbent Sheet Extraction Prior to Direct Analysis in Real-Time Mass Spectrometry (DART-MS).
J Agric Food Chem
October 2021
Department of Food Science, Cornell University, Stocking Hall, Ithaca, New York 14853, United States.
Poly(dimethylsiloxane)-based thin-film sorbent sheets (SPMESH) have previously been used for parallel headspace (HS) extraction prior to direct analysis in real-time mass spectrometry (DART-MS) for rapid quantitation of odorants in complex matrices. However, HS-SPMESH extraction is poorly suited for less volatile odorants, e.g.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!