lambda gua transducing bacteriophages were used to identify and sequence the secondary attachment site for lambda in the guaB gene of Escherichia coli. The sequence matched the primary core sequence at nine positions, and a putative integrase binding-site overlapped the left core-arm junction. Recombinational crossover occurred between nucleotides -3 and +2 of the core region.
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http://dx.doi.org/10.1128/jb.168.2.1048-1050.1986 | DOI Listing |
Nat Prod Res
January 2025
Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, P. R. China.
A new hydrindane derivative, asperhydrindane A (), along with two known sterol analogues [isocyathisterol () and ganodermasides D ()] were isolated from the mangrove-derived fungus GXIMD 03158 attaching to the mangrove L. The structure of was elucidated based on extensive spectral analysis, HRESIMS, and calculated ECD methods. All compounds were evaluated for antibacterial activity.
View Article and Find Full Text PDFMolecules
January 2025
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, SK-845 38 Bratislava, Slovakia.
Phenylethanoid glycosides (PhGs) are widely occurring secondary metabolites of medicinal plants with interesting biological activities such as antioxidant, anti-inflammatory, neuroprotective, antiviral, hepatoprotective, immunomodulatory, etc. They are characterized by a structural core formed by a phenethyl alcohol, usually tyrosol or hydroxytyrosol, attached to β-D-glucopyranose via a glycosidic bond. This core is usually further decorated by attached phenolic acids or another saccharide.
View Article and Find Full Text PDFPLoS One
January 2025
Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
This study identifies the secondary metabolites from Alternaria alternate and evaluates their ACE-2: Spike RBD (SARS-CoV-2) inhibitory activity confirmed via immunoblotting in human lung microvascular endothelial cells. In addition, their in vitro anti-inflammatory potential was assessed using a cell-based assay in LPS-treated RAW 264.7 macrophage cells.
View Article and Find Full Text PDFSci Total Environ
January 2025
Department of Geosciences & Natural Resource Management, Geology, University of Copenhagen, Denmark.
Due to increasing plastic production, the continuous release of primary and secondary nanoplastic particles (NPs, <1 μm) has become an emerging contaminant in terrestrial environments. The fate and transport of NPs in subsurface environments remain poorly understood, largely due to the complex interplay of mineralogical, chemical, biological, and morphological heterogeneity. This study examines interactions between abundant subsurface minerals and NPs under controlled water chemistry (1 mM KCl, pH 5.
View Article and Find Full Text PDFBioconjug Chem
January 2025
Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, U.K.
Lipidated analogues of glucagon-like peptide 1 (GLP-1) have gained enormous attention as long-acting peptide therapeutics for type 2 diabetes and also antiobesity treatment. Commercially available therapeutic lipidated GLP-1 analogues, semaglutide and liraglutide, have the great advantage of prolonged half-lives of hours and days instead of minutes as is the case for native GLP-1. A crucial factor in the development of novel lipidated therapeutic peptides is their physical stability, which greatly influences manufacturing and drug product development.
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