University of Belgrade, Faculty of Biology, Studentski trg 3, Belgrade, Serbia. Electronic address:
Published: October 2017
AI Article Synopsis
Article Abstract
Cannabis sativa subspecies, known as industrial hemp (C. sativa sativa) and marijuana (C. sativa indica) show no evident morphological distinctions, but they contain different levels of psychoactive Δ-9-tetrahidrocanabinol (THC), with considerably higher concentration in marijuana than in hemp. C. sativa subspecies differ in sequence of tetrahydrocannabinolic acid (THCA) synthase gene, responsible for THC production, and only one active copy of the gene, distinctive for marijuana, is capable of producing THC in concentration more then 0,3% in dried plants, usually punishable by the law. Twenty different samples of marijuana that contain THC in concentration more then 0,3% and three varieties of industrial hemp were analyzed for presence of an active copy of THCA synthase gene using in-house developed restriction fragment length polymorphism (RFLP) method All twenty samples of marijuana were positive for the active copy of THCA synthase gene, 16 of them heterozygous. All three varieties of industrial hemp were homozygous for inactive copy. An algorithm for the fast and accurate forensic analysis of samples suspected to be marijuana was constructed, answering the question if an analyzed sample is capable of producing THC in concentrations higher than 0.3%.
Hemp Cheungsam, a non-drug Cannabis sativa strain, has low levels of the psychoactive compound THCA and its cannabinoid biosynthesis genes were not previously studied.
RNA-sequencing was used to analyze flower, leaf, and stem tissues of hemp Cheungsam, revealing that cannabinoid biosynthesis mainly occurs in the flowers, with a strong emphasis on CBDA production over THCA.
The study confirms that flowers are the primary site for cannabinoid production, with high expression of CBDA synthase and suggests that key genes involved in cannabinoid biosynthesis have identifiable orthologs in hemp Cheungsam.
Euromed Research Center, Euromed Faculty of Pharmacy, School of Engineering in Biomedical and Biotechnology, Euromed University of Fes (UEMF), Meknes Road, Fez 30000, Morocco.
- This study explored the genetic, chemical, and biological properties of ten traditional Moroccan seed varieties, identifying two unique molecular profiles through DNA analysis and discovering a new gene sequence related to THCA synthase.
- Chemical analysis revealed a total of 37 compounds, including 13 non-volatile chemicals (like inactive cannabinoids and polyphenols) and 24 volatile compounds, with 7 of these being newly identified in seed extracts.
- The research also assessed the seeds' biological activities, showcasing their antioxidant, antimicrobial, cytotoxic, and potential antiviral effects, suggesting these seeds could be valuable for future nutritional and health-related studies.
L. has been widely used by humans for centuries for various purposes, such as industrial, ceremonial, medicinal, and food. The bioactive components of L.
Aim: Pseudouridylation has demonstrated the potential to control the development of numerous malignancies. PUS7(Pseudouridine Synthase 7) is one of the pseudouridine synthases, but the literature on this enzyme is limited to several cancer types. Currently, no investigation has been performed on the systematic pan-cancer analysis concerning PUS7 role in cancer diagnosis and prognosis.
Introduction: Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system characterized by neuroinflammation, demyelination and axonal loss. Cannabis, an immunomodulating agent, is known for its ability to treat MS effectively. However, due to variations in the profile of secondary metabolites, especially cannabinoids, among cannabis cultivars, the effectiveness of cannabis treatment can vary, with significant variability in the effects on different biological parameters.
