AI Article Synopsis
- A study was conducted to improve the determination of drowning as a cause of death by investigating the presence of aquatic bacteria and diatoms in deceased individuals found in water.
- Researchers utilized temperature gradient gel electrophoresis (TGGE) on samples from water at the site, blood, and liver from 27 bodies labeled as drowning deaths to find matches in 16S rDNA.
- The results showed a higher correspondence in the drowned bodies compared to older methods, and TGGE is considered a safer alternative for diagnosing drowning.
Article Abstract
When a body is discovered in water, it is difficult to conclude whether the cause of death was drowning, even today. Although diatom testing by the digestive method is classical, we hypothesized that aquatic bacteria, as well as diatoms, might be detected in drowned bodies, and conducted temperature gradient gel electrophoresis (TGGE)-targeting 16S rDNA. DNA was extracted from the site water, and from heart blood and liver samples from 27 bodies concluded as drowning deaths by autopsy and subjected to TGGE after amplification of 16S rDNA by polymerase chain reaction. We observed whether the feature point of each 16S rDNA from the site water and blood or liver samples matched. Considerably higher correspondence was observed in drowned bodies, and the rate was higher than that achieved with the digestive method. Moreover, TGGE is safer than the digestive method. Our study suggests that this method can aid diagnosis of drowning.
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| http://dx.doi.org/10.1111/1556-4029.13606 | DOI Listing |
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