AI Article Synopsis
- A new two-photon fluorescent probe (J1) was developed to selectively stain nucleolar RNA, identified from a series of three similar compounds (J1-J3).
- The compounds were tested for their optical properties, showing significant Stokes shifts and two-photon absorption capabilities in the near-infrared region, which supports the use of J1 for specific imaging.
- J1 demonstrated high selectivity for binding to nucleolar RNA compared to cytosolic RNA, making it a promising tool for visualizing rRNA in living cells through various experimental methods.
Article Abstract
A two-photon fluorescent probe (J1) that selectively stains intracellular nucleolar RNA was screened from three water-soluble terpyridine derivatives (J1-J3) with quaternary ammonium groups. The photophysical properties of J1-J3 were systemically investigated both experimentally and theoretically, revealing that J1-J3 possess large Stokes shifts and the two-photon absorption action cross sections range from 38 to 97 GM in the near-infrared region. This indicates that J1 could specifically stain nucleoli by targeting the nucleolar rRNA from the recognition experiments in vitro, the two-photon imaging experiments, and the stimulated emission depletion in vivo. The mechanism of action in which J1 binds to the nucleolar rRNA was researched via both experiments and molecular modeling. The high binding selectivity of J1 to nucleolar RNA over cytosolic RNA made this probe a potential candidate to visualize rRNA probe in the living cells.
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| http://dx.doi.org/10.1021/acsami.7b08068 | DOI Listing |
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