One of the main issues when using traveling wave ion mobility spectrometry (TWIMS) for the determination of collisional cross-section (CCS) concerns the need for a robust calibration procedure built from referent ions of known CCS. Here, we implement synthetic polymer ions as CCS calibrants in positive ion mode. Based on their intrinsic polydispersities, polymers offer in a single sample the opportunity to generate, upon electrospray ionization, numerous ions covering a broad mass range and a large CCS window for different charge states at a time. In addition, the key advantage of polymer ions as CCS calibrants lies in the robustness of their gas-phase structure with respect to the instrumental conditions, making them less prone to collisional-induced unfolding (CIU) than protein ions. In this paper, we present a CCS calibration procedure using sodium cationized polylactide and polyethylene glycol, PLA and PEG, as calibrants with reference CCS determined on a home-made drift tube. Our calibration procedure is further validated by testing the polymer calibration to determine CCS of numerous different ions for which CCS are reported in the literature. Graphical Abstract ᅟ.
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Cell Commun Signal
January 2025
Beijing An Zhen Hospital, Capital Medical University, The Key Laboratory of Remodeling Cardiovascular Diseases, Ministry of Education; Collaborative Innovation Center for Cardiovascular Disorders, Beijing Institute of Heart Lung and Blood Vessel Disease, Beijing, 100029, China.
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January 2025
Department of Physics, Punjab Engineering College (Deemed to be University), Chandigarh, 160012, India.
Rapid and accurate detection of Escherichia coli (E. coli) is critical for maintaining water quality, and protecting aquatic ecosystems and public health. This research focuses on the development of a Förster resonance energy transfer (FRET)-based "turn-on" fluorescent nanosensor for real time, sensitive detection of E.
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December 2024
Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, 230026, P. R. China.
Ferroptosis, a non-apoptotic form of cell death characterized by the production of reactive oxygen species (ROS) and massive accumulation of lipid peroxidation (LPO), shows significant promise in cancer therapy. However, the overexpression of glutathione (GSH) at the tumor site and insufficient ROS often result in unsatisfactory therapeutic efficacy. A multistage, GSH-consuming, and ROS-providing carrier-free nanodrug capable of efficiently loading copper ions (Cu), sorafenib (SRF), and chlorogenic acid (CGA) (Cu-CGA-SRF, CCS-NDs) is developed to mediate enhanced ferroptosis therapy.
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December 2024
Department of Physics, Punjab Engineering College (Deemed to be University), Chandigarh 160012, India.
Mercury (Hg) has been recognized as a global pollutant with a toxic, mobile, and persistent nature. It adversely affects the ecosystem and human health. Already developed biosensors for Hg detection majorly suffer from poor sensitivity and specificity.
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December 2024
Department of Chemistry, COBS&H, CCS Haryana Agricultural University Hisar-125004 Haryana India.
The combination of 4,4,4-trifluoro-1-phenyl-1,3-butanedione (TFPB) and pyrazine (pyz) with Tb ions forms two distinct types of complexes, represented by the formulas [Tb(TFPB)(L)], where L is either HO or pyz, and [(Tb(TFPB))pyz]. A detailed examination of the impact of the surrounding environment on the photophysical properties of these synthesized complexes was conducted. Photoluminescence (PL) analysis indicated that the magnetic dipole transition (D → F) is dominant in Tb(iii)-based systems.
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