Annexin A1 (ANXA1) down-regulation is an early and frequent event in the development of head and neck squamous cell carcinomas (HNSCC). In an attempt to identify the underlying mechanisms of reduced ANXA1 protein expression, this study investigated ANXA1 mRNA expression in HNSCC specimens by both in situ hybridization and RT-qPCR. Results showed a perfect concordance between the pattern of ANXA1 mRNA and protein detected by immunofluorescence in tumors, precancerous lesions and normal epithelia, reflecting that ANXA1 down-regulation occurs at transcriptional level. We also found that both miR-196a and miR-196b levels inversely correlated with ANXA1 mRNA levels in paired HNSCC tissue samples and patient-matched normal mucosa. In addition, endogenous levels of ANXA1 mRNA and protein were consistently and significantly down-regulated upon miR-196a and miR-196b over-expression in various HNSCC-derived cell lines. The direct interaction of both mature miR-196a and miR-196b was further confirmed by transfection with Anxa1 3'UTR constructs. Combined bioinformatics and functional analysis of ANXA1 promoter activity contributed to identify key regions and potential mediators of ANXA1 transcriptional control. This study unveils that, in addition to miR-196a, miR-196b also directly targets ANXA1 in HNSCC.
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http://dx.doi.org/10.1038/s41598-017-07169-w | DOI Listing |
Eur J Med Res
December 2024
Department of Ophthalmology, Zhongshan City People's Hospital, Zhongshan, Guangdong, China.
Background: Age-related macular degeneration (AMD), is a neurodegenerative ocular disease. This study investigated the role of ferroptosis-related genes and their interaction with immune cell infiltration in AMD.
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Open Med (Wars)
November 2024
Department of Hematology, West China Hospital, Sichuan University, #37 Guo Xue Xiang Street, Chengdu, 610041, China.
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View Article and Find Full Text PDFDiscov Oncol
November 2024
Department of Urology, Jinzhou Medical University, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.
Background: Apoptosis and apoptotic genes play a critical role in the carcinogenesis and progression of bladder cancer. However, there is no prognostic model established by apoptotic genes.
Methods: Messenger RNA (mRNA), Expression data, and related clinical data were obtained from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database.
Front Chem
October 2024
College of Clinical Medicine, Shandong Second Medical University, Weifang, China.
Background: Osteoarthritis (OA) is the most common joint disease, which mainly damages articular cartilage and involves the whole joint tissue. It has the characteristics of long course, repeated symptoms and high disability rate, and the incidence trend is gradually increasing. Tetramethylpyrazine (TMP) is the main alkaloid active substance in Ligusticum wallichii, a traditional Chinese medicine, which has the effect of promoting blood circulation and dredging collaterals, and has a good effect on the treatment of early OA, but its molecular mechanism has not been fully clarified so far.
View Article and Find Full Text PDFPoult Sci
December 2024
Department of Poultry Science, University of Georgia, 120 D.W. Brooks Drive, Athens, GA 30602, USA. Electronic address:
Caused by the Gram-positive bacteria Clostridium perfringens, necrotic enteritis (NE) is an enteric disease with significant economic implications in broiler production. This study employed an experimental NE model involving co-infection with Eimeria maxima and C. perfringens to assess whether sulfate polysaccharides extracted from marine macroalgae could mitigate the adverse effects of NE in broilers.
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