AI Article Synopsis
- Salicylic acid (SA) is crucial for plant defense mechanisms, and its response involves proteins NPR1 and NRB4 in Arabidopsis.
- Researchers identified various protein interactors of NRB4, specifically β-carbonic anhydrases (βCAs), and noted that certain βCAs also interacted with NPR1 in a SA-dependent way.
- The study revealed that mutations in multiple βCA genes can lead to partial insensitivity to SA, indicating that βCAs play a role in how plants perceive this important hormone.
Article Abstract
The plant hormone salicylic acid (SA) is required for defense responses. NON EXPRESSER OF PATHOGENESIS RELATED 1 (NPR1) and NON RECOGNITION OF BTH-4 (NRB4) are required for the response to SA in Arabidopsis (Arabidopsis thaliana). Here, we isolated several interactors of NRB4 using yeast two-hybrid assays. Two of these interactors, βCA1 and βCA2, are β-carbonic anhydrase family proteins. Since double mutant βca1 βca2 plants did not show any obvious phenotype, we investigated other βCAs and found that NRB4 also interacts with βCA3 and βCA4. Moreover, several βCAs interacted with NPR1 in yeast, including one that interacted in a SA-dependent manner. This interaction was abolished in loss-of-function alleles of NPR1. Interactions between βCAs and both NRB4 and NPR1 were also detected in planta, with evidence for a triple interaction, NRB4-βCA1-NPR1. The quintuple mutant βca1 βca2 βca3 βca4 βca6 showed partial insensitivity to SA. These findings suggest that one of the functions of carbonic anhydrases is to modulate the perception of SA in plants.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5533460 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0181820 | PLOS |
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