Despite the well-known toxic effect of chloride sodium (NaCl), only few studies analyze its genotoxic effect on crops. Micronucleus assay is an efficient tool for the measure of chromosomal DNA damages. Based on this test, the effects of salt on micronucleus induction, and on chromosome and nucleus changes, were investigated in Vicia faba root tips treated with NaCl (electrical conductivity = 25 dS/m) during 24 hours. V. faba meristematic cells showed a significant increase in the number of micronuclei compared with the control. Micronucleus size varied from small to large and appeared at different stages of mitosis. Moreover, different chromosomal aberrations like breaks, bridges, rings, vagrant, and lagging chromosomes, were observed in the metaphase, the anaphase, and the telophase. In addition, nuclear alterations in the interphase were characterized by the presence of binucleated cells, strap nuclei with lesions, hyperchromasia, buds, and nuclei with irregular shapes and lesions.

Download full-text PDF

Source
http://dx.doi.org/10.2175/106143017X14902968254827DOI Listing

Publication Analysis

Top Keywords

nuclear alterations
8
chromosomal nuclear
4
alterations root
4
root cells
4
cells induced
4
induced sodium
4
sodium chloride
4
chloride  
4
  despite
4
despite well-known
4

Similar Publications

Glioprotective Effects of Resveratrol Against Glutamate-Induced Cellular Dysfunction: The Role of Heme Oxygenase 1 Pathway.

Neurotox Res

January 2025

Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Resveratrol, a natural polyphenol, has shown promising neuroprotective effects in several in vivo and in vitro experimental models. However, the mechanisms by which resveratrol mediates these effects are not fully understood. Glutamate is the major excitatory neurotransmitter in the brain; however, excessive extracellular glutamate levels can affect neural activity in several neurological diseases.

View Article and Find Full Text PDF

Ameliorative impact of sacubitril/valsartan on paraquat-induced acute lung injury: role of Nrf2 and TLR4/NF-κB signaling pathway.

Naunyn Schmiedebergs Arch Pharmacol

January 2025

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.

Herbicides such as paraquat (PQ) are frequently utilized particularly in developing nations. The present research concentrated on the pulmonary lesions triggered by PQ and the beneficial effect of the angiotensin receptor neprilysin inhibitor (ARNI), sacubitril/valsartan, against such pulmonary damage. Five groups of rats were established: control, ARNI, PQ (10 mg/kg), ARNI 68 + PQ, and ARNI 34 + PQ.

View Article and Find Full Text PDF

Historically considered downstream effects of tumorigenesis-arising from changes in DNA content or chromatin organization-nuclear alterations have long been seen as mere prognostic markers within a genome-centric model of cancer. However, recent findings have placed the nuclear envelope (NE) at the forefront of tumor progression, highlighting its active role in mediating cellular responses to mechanical forces. Despite significant progress, the precise interplay between NE components and cancer progression remains under debate.

View Article and Find Full Text PDF

Cyclooxygenase 2 overexpression suppresses Smad3 and augments ERK1/2 signaling activated by TGFβ1 in endometrial stromal cells: a novel insight into endometriosis pathogenesis.

Mol Cell Endocrinol

January 2025

The Gynecology Department, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. Electronic address:

Research Question: To investigate the underlying mechanisms driving the opposing effects of transforming growth factor-beta 1 (TGFβ1) on the proliferation of control (CESCs) and ectopic (EESCs) endometrial stromal cells.

Design: Cell proliferation assays (CCK-8 and colony formation) were employed to assess the effects of TGFβ1 on CESC and EESC proliferation. An immortalized human endometrial stromal cell line (HESC) was used to elucidate the mechanisms behind cytostatic effect of TGFβ1 and the potential role of cyclooxygenase (COX)-2 in mediating the modulation of TGFβ1 signaling.

View Article and Find Full Text PDF

Mina53 catalyzes arginine demethylation of p53 to promote tumor growth.

Cell Rep

January 2025

Ministry of Education Key Laboratory of Biosystems Homeostasis & Protection, College of Life Sciences, Zhejiang University, Hangzhou, China; Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang Provincial Key Laboratory of Pancreatic Disease, School of Medicine, Zhejiang University, Hangzhou, China; Cancer Center, Zhejiang University, Hangzhou, China. Electronic address:

Arginine methylation is a common post-translational modification that plays critical roles in many biological processes. However, the existence of arginine demethylases that remove the modification has not been fully established. Here, we report that Myc-induced nuclear antigen 53 (Mina53), a member of the jumonji C (JmjC) protein family, is an arginine demethylase.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!