Department of Biotechnology, School of Engineering and Technology, Sharda University, Knowledge Park III, Greater Noida, U.P, India.
Published: December 2017
AI Article Synopsis
Article Abstract
Enterohemorrhagic Escherichia coli (E. coli) O157:H7, a gram-negative bacteria identified as a foodborne pathogen causing severe disease is of great concern worldwide. The pathogenicity of E. coli O157:H7 is due to the presence of some virulence factors and its ability to form biofilm which resist antimicrobial compounds, withstand harsh environmental condition and protects from the host immune responses. Formation of biofilm is a multistep process such as adhesion, cellular aggregation and productions of extracellular matrix in which colonies are embedded. There are high numbers of research in the discovery of natural and synthetic compounds which can attenuate the E. coli O157:H7 biofilm formation as well as suppress virulence-related genes. The present review article focuses on the steps involved in E. coli O157:H7 biofilm formation, factors associated with virulence and attenuation.
This study evaluated how a coating made from nanochitosan and whey protein isolate, combined with summer savory essential oil and oxygen absorber packaging, affects the growth of specific bacteria on rainbow trout fillets stored in the fridge.
Results showed that while Listeria monocytogenes initially decreased in all treatment groups, it eventually increased, indicating limited effectiveness of the treatments against this bacterium.
In contrast, the combined treatment with the coating, essential oil, and oxygen absorber was most effective in slowing the growth of E. coli and reducing Pseudomonas aeruginosa levels compared to other methods.
* Out of 544 samples analyzed from 272 bovine carcasses, 11 tested positive for O157:H7 (4.0%), and 6 of those had specific pathogenicity genes detected.
* Additionally, 22 carcasses (8.0%) tested positive for the unspecified pathogen, but none contained the specific genes associated with pathogenicity.
To evaluate the typing and clinical application effect based on clustered regularly interspaced short palindromic repeats (CRISPRs), serotype, and Multilocus Sequence Typing (MLST). The spacers, serotype and sequence type (ST) were obtained with CRISPRsFinder, SeroTypeFinder and MLST. PCR was used to amplify the CRISPRs, and the spacers were used to predict serotype and ST, then comparing with the serotype and ST.
Rapid measurement of waterborne bacterial viability is crucial for ensuring the safety of public health. Herein, we proposed a colorimetric assay for rapid measurement of waterborne bacterial viability based on a difunctional gold nanoprobe (dGNP). This versatile dGNP is composed of bacteria recognizing parts and signal indicating parts, and can generate color signals while recognizing bacterial suspensions of different viabilities.
Antibiotic resistance is a major global health issue, prompting researchers to seek alternative treatment methods.
Molasses shows promising antimicrobial properties that may help combat resistant bacteria by disrupting bacterial cell membranes and proteins.
The study found that although molasses did not work synergistically with phages, it increased the size of phage plaques and enhanced their activity, suggesting potential benefits of using molasses alongside phage therapy.
