AI Article Synopsis
- Osteosarcoma (OS) is the most common malignant bone tumor in young individuals, and the long non-coding RNA ZFAS1 is found to be significantly overexpressed in OS cases, linked to poor outcomes.
- ZFAS1 promotes OS cell growth and metastasis by binding to specific microRNAs (miR-200b and miR-200c) and upregulating the BMI1 gene, while also interacting with the ZEB2 protein to enhance its stability.
- SP1 is identified as a key factor activating ZFAS1, suggesting that targeting ZFAS1 could be a promising approach for treating OS.
Article Abstract
Osteosarcoma (OS) is the most common malignant bone tumor in children and adolescents. LncRNAs have recently gained widespread attention and have been shown to have crucial roles in various biological regulatory processes. ZFAS1, a newly identified lncRNA, was shown to be dysregulated in several cancers. However, little is known about the alteration and functional significance of ZFAS1 in OS. In the present study, for the first time, we revealed a functional role of ZFAS1 on OS growth and metastasis. The expression of ZFAS1 was significantly overexpressed in OS samples and cell lines, and upregulation of ZFAS1 is significantly associated with unfavorable prognosis of OS patients. Functional assays also demonstrated that ZFAS1 enhanced the growth and metastatic ability of OS cells and . Mechanistically, we found that ZFAS1 positively regulated malignant phenotypes by competitively binding the miR-200b and miR-200c and upregulating BMI1. ZFAS1 also interacted with ZEB2 and regulated ZEB2 protein stability. Furthermore, we demonstrated that SP1 functions as an upstream activated factor of ZFAS1. ZFAS1 may be a potential therapeutic target for OS tumorigenesis and progression.
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