The roles of NF-κB and ROS in regulation of pro-inflammatory mediators of inflammation induction in LPS-stimulated zebrafish embryos.

Fish Shellfish Immunol

Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Republic of Korea; Department of Marin Biotechnology, University of Science and Technology, Daejeon 34113, Republic of Korea. Electronic address:

Published: September 2017

AI Article Synopsis

  • The study investigated how reactive oxygen species (ROS) and the protein NF-κB contribute to inflammation in zebrafish embryos stimulated with lipopolysaccharide (LPS).
  • LPS exposure led to higher levels of nitric oxide (NO) and ROS, as well as increased expression of inflammatory proteins iNOS and COX-2 in the embryos compared to a control group.
  • The inhibitors N-acetyl-l-cysteine (NAC) and pyrrolidine dithiocarbamate (PDTC) effectively reduced NO and ROS production and the inflammatory markers, showing that both ROS and NF-κB play key roles in the inflammation process induced by LPS.

Article Abstract

In this study, the roles of reactive oxygen species (ROS) and NF-κB on inflammation induction in lipopolysaccharide (LPS)-stimulated zebrafish embryos were evaluated using N-acetyl-l-cysteine (NAC) and pyrrolidine dithiocarbamate (PDTC), specific inhibitors of ROS and NF-κB, respectively. LPS-stimulated zebrafish embryos showed increasing production of NO and ROS and expression of iNOS and COX-2 protein, compared to a control group without LPS. However, NAC significantly inhibited production of NO and ROS and markedly suppressed expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. The mRNA expressions of NF-κB such as p65NF-κB and IκB-A were significantly increased after LPS stimulation, whereas PDTC attenuated mRNA expression of NF-κB. PDTC also inhibited production of NO and reduced expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. Taken together, these results indicated that LPS increases pro-inflammatory mediators in zebrafish embryos through ROS and NF-κB regulation.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fsi.2017.07.041DOI Listing

Publication Analysis

Top Keywords

zebrafish embryos
24
lps-stimulated zebrafish
20
ros nf-κb
12
expression inos
12
inos cox-2
12
cox-2 protein
12
pro-inflammatory mediators
8
inflammation induction
8
production ros
8
inhibited production
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!