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Immobilization of purified pectinase from Aspergillus nidulans on chitosan and alginate beads for biotechnological applications.
Microb Cell Fact
January 2025
Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.
Background: Because the process is cost-effective, microbial pectinase is used in juice clearing. The isolation, immobilization, and characterization of pectinase from Aspergillus nidulans (Eidam) G. Winter (AUMC No.
View Article and Find Full Text PDFBiosynthesis and health promoting traits of green synthesized cobalt oxide nanoparticles.
Sci Rep
January 2025
Obstetrics and Gynaecology Department, Faculty of Medicine, Minia University, Minia, Egypt.
Nanomedical applications have increased significantly. This work aimed to fabricate and characterize cobalt oxide nanoparticles (CoOnps) synthesized biologically via aqueous Alhagi maurorum extract and evaluate their cytotoxic and antimicrobial impacts. Green-synthesized CoOnps were prepared and analyzed using UV-Vis spectrophotometer UV-vis, Scanning electron microscopy (SEM), Transmission electron microscopy TEM, Energy dispersive X-ray analysis EDAX, Fourier transform infrared, FTIR, and X-ray diffraction (XRD).
View Article and Find Full Text PDFPhytochemical profile and in vitro evaluation of cassava (Manihot esculenta Crantz) foliage as ruminant feed with/without green banana flour.
Sci Rep
January 2025
Animal Production Department, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt.
Cassava (Manihot esculenta Crantz) is a crucial crop in tropics and subtropics, primarily cultivated for its tuber. However, its foliage is rich in protein and can supply essential elements for ruminants. The objective of this study was to evaluate the phytochemical compounds by Gas chromatography-MS (GC-MS) and the main phenolic by High Pressure Liquid Chromatography (HPLC) present in cassava foliage, along with the fermentation pattern using a semi-automated gas production (GP) system.
View Article and Find Full Text PDFRisk assessment and monitoring of tranquilizers in live seafood: Analysis of natural essential oil and 2-phenoxyethanol by GC-MS/MS.
Sci Total Environ
January 2025
Division of Civil Environmental Engineering, Pusan National University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea; Institute for Environmental and Energy, Pusan National University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan, 46241, Republic of Korea. Electronic address:
An analytical method for nine tranquilizer compounds, including eight major natural oil components and 2-phenoxyethanol, was developed using gas chromatography tandem mass spectrometry and validated under CODEX and National Institute of Food and Drug Safety Evaluation guidelines. Subsequently, 315 live seafood samples under Korean preference for sashimi (eight species) were monitored with sales distribution channels and place of origin. A total of 76 detection cases in 65 seafood samples contained the target tranquilizer compounds at concentrations ranging from 2.
View Article and Find Full Text PDFBio-inspired chitosan/polydopamine-nanoparticle based sorbent bead: A versatile platform for separation and HPLC analysis of tetracycline antibiotics from various sample matrix.
J Food Drug Anal
December 2024
Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan.
This study introduces an innovative bio-based sorbent bead crafted by integrating chitosan (CS) biopolymers, Fe(NO3)3 and polydopamine nanoparticles (PDA NPs) via glutaraldehyde crosslinking. The primary focus of this study was the concurrent separation of diverse tetracycline antibiotics (TCs), followed by rigorous reversed-phase liquid chromatography analysis. The fabricated CS/Fe@PDA sorbent beads were comprehensively characterized using scanning electron microscopy and energy-dispersive X-ray spectroscopy, revealing a surface rich in active carbon (C), nitrogen (N), and oxygen (O) moieties.
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