Diabetic cardiomyopathy has been systematically associated with compromised mitochondrial energetics and increased generation of reactive oxygen species (ROS) that underlie its progression to heart failure. Methylene blue is a redox drug with reported protective effects mainly on brain mitochondria. The purpose of the present study was to characterize the effects of acute administration of methylene blue on mitochondrial respiration, HO production, and calcium sensitivity in rat heart mitochondria isolated from healthy and 2 months (streptozotocin-induced) diabetic rats. Mitochondrial respiratory function was assessed by high-resolution respirometry. HO production and calcium retention capacity were measured spectrofluorimetrically. The addition of methylene blue (0.1 μmol·L) elicited an increase in oxygen consumption of mitochondria energized with complex I and II substrates in both normal and diseased mitochondria. Interestingly, methylene blue elicited a significant increase in HO release in the presence of complex I substrates (glutamate and malate), but had an opposite effect in mitochondria energized with complex II substrate (succinate). No changes in the calcium retention capacity of healthy or diabetic mitochondria were found in the presence of methylene blue. In conclusion, in cardiac mitochondria isolated from diabetic and nondiabetic rat hearts, methylene blue improved respiratory function and elicited a dichotomic, substrate-dependent effect on ROS production.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1139/cjpp-2017-0074 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Two-dimensional porous porphyrin materials composed of robust tin(IV)-porphyrin linkages for photocatalytic wastewater remediation.
Dalton Trans
January 2025
Department of Chemistry and Bioscience, Kumoh National Institute of Technology, Gumi 39177, Republic of Korea.
Porphyrin-based two-dimensional porous materials (SnP-H2TCPP, SnP-ZnTCPP) composed of robust Sn(IV)-porphyrin linkages have been synthesized by reacting -dihydroxo[5,10,15,20-tetraphenylporphyrinato]tin(IV) (SnP) with [5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin] (HTCPP) and [5,10,15,20-tetrakis(4-carboxyphenyl)porphyrinato]zinc(II) (ZnTCPP), respectively. The strength of the interaction between the carboxylic acid group of the monomeric porphyrins (HTCPP and ZnTCPP) and the axial hydroxyl moiety of SnP enables the construction of highly stable framework materials, which were characterized by FT-IR, UV-vis, and emmission spectroscopy, powder XRD, elemental analysis, and thermogravimetric analysis (TGA). SnP-H2TCPP and SnP-ZnTCPP absorb visible light strongly over a wide range, demonstrating weak perturbation in the electronic ground state structures of the π-conjugated aromatic moieties compared to the starting monomeric units.
View Article and Find Full Text PDFCompetitive Langmuir Model for Dye Mixture Adsorption: Disagreement between Theoretical Ground and Application Condition.
Langmuir
January 2025
Center for Environmental Process Engineering, Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh.
Precise prediction of adsorption in a multicomponent system is vital for successful design of dye-contaminated industrial wastewater treatment processes. The present work looks for the reason behind the failure of the competitive Langmuir model (CLM) to describe adsorption in such systems, while the Langmuir model (LM) successfully describes the process for a single dye solution. With that end, derivations of LM and CLM have been revisited, and a criterion for the universality of active sites has been defined.
View Article and Find Full Text PDFAptamer-based DNAzyme walker electrochemical biosensing strategy for Acinetobacter baumannii detection.
Bioelectrochemistry
December 2024
Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, 25 Taiping Street, Luzhou 646000, Sichuan, China; Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, China; Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, Sichuan, China. Electronic address:
In this study, an innovative electrochemical biosensor was developed for the rapid, specific, and sensitive detection of Acinetobacter baumannii without the need for sample pretreatment. The biosensor utilized an aptamer as a specific capture probe for A. baumannii and employed a self-powered DNAzyme walker cleavage cycle reaction to achieve signal amplification.
View Article and Find Full Text PDFAn aptasensor consisting of the integrated MOF-derived Zn-MOF-on-Co-MOF coupled with AuNPs nanocomposites and aptamer for ultrasensitive assay of fipronil.
Food Chem
December 2024
School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
The conductivity of Zn-MOF-on-Co-MOF synthesized by one-pot method is improved by searching for the optimum carbonization temperature, which overcomes the limitation of traditional MOF. In order to further enhance electron transfer, the mesoporous PtPdCo trimetal was introduced, which provided considerable load capacity for methylene blue (MB) and reverse complementary DNA (sDNA), and also showed excellent catalytic activity for MB. In this study, the conductivity of aptasensor was improved by modifying carbonized MOF as the base material.
View Article and Find Full Text PDFGreener Synthesis of Poly(LIM--DVB--AMPS): A Sustainable Approach to Methylene Blue Removal.
ACS Omega
December 2024
Faculty of Science, Department of Chemistry, Ege University, İzmir 35100, Turkey.
A novel environmentally friendly adsorbent, poly(limonene--divinylbenzene--2-acrylamido-2-methyl-1-propanesulfonic acid, LIM--DVB--AMPS), was synthesized and applied for the adsorption of methylene blue from aqueous solutions in this study. The structure, morphology, and thermal stability of the green adsorbent were determined by the FTIR, SEM, TGA/DTA/DTG, and BET techniques, ζ potential, and elemental analysis. The efficiency of the adsorption process was improved with respect to several experimental conditions, viz.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!