Ethnopharmacological Relevance: Acanthopanax koreanum (Araliaceae) has been used in traditional medicine for enhancing vitality, rheumatism, and bone-related pains. But its activity on cartilage protection has not been known yet.
Aim Of The Study: Matrix metalloproteinase (MMP)-13 has an important role in degrading cartilage materials under pathologic conditions such as arthritis. The present study was designed to find the inhibitory activity of impressic acid on MMP-13 expression and cartilage protective action.
Materials And Methods: 70% ethanol extract of Acanthopanax koreanum leaves and impressic acid, a major constituent isolated from the same plant materials, were examined on MMP-13 down-regulating capacity in IL-1β-treated human chondrocyte cell line (SW1353) and rabbit cartilage explants.
Results: In IL-1β-treated SW1353 cells, impressic acid significantly and concentration-dependently inhibited MMP-13 expression at 0.5-10μM. Impressic acid was found to be able to inhibit MMP-13 expression by blocking the phosphorylation of signal transducer and activator of transcription-1/-2 (STAT-1/-2) and activation of c-Jun and c-Fos among the cellular signaling pathways involved. Further, impressic acid was found to inhibit the expression of MMP-13 mRNA (47.7% inhibition at 10μM), glycosaminoglycan release (42.2% reduction at 10μM) and proteoglycan loss in IL-1-treated rabbit cartilage explants culture. In addition, a total of 21 lupane-type triterpenoids structurally-related to impressic acid were isolated from the same plant materials and their suppressive activities against MMP-13 expression were also examined. Among these derivatives, compounds 2, 3, 16, and 18 clearly down-regulated MMP-13 expression. However, impressic acid was more potent than these derivatives in down-regulating MMP-13 expression.
Conclusions: Impressic acid, its related triterpenoids, and A. koreanum extract have potential as therapeutic agents to prevent cartilage degradation by inhibiting matrix protein degradation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jep.2017.07.034 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antiosteosarcoma effects of novel 23-nor-3,4-seco-3-acetallupane triterpenoids from Acanthopanax gracilistylus W.W. Smith var. gracilistylus in 143B cells.
Fitoterapia
April 2022
School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
Three unprecedented 23-nor-3,4-seco-3-acetallupane triterpenoids, gracilistylacid A-C (1-3), along with three known lupanoids (4-6), were isolated from the aerial parts of Acanthopanax gracilistylus W.W. Smith var.
View Article and Find Full Text PDFAcankoreagenin and acankoreosides, a family of lupane triterpenoids with anti-inflammatory properties: an overview.
Ann N Y Acad Sci
October 2021
OncoWitan, Lille (Wasquehal), Wasquehal, France.
Acankoreagenin (ACK, also known as acankoreanogenin and HLEDA) and impressic acid are two lupane-type triterpenes that can be isolated from various Acanthopanax and Schefflera species. They efficiently block activation of the NF-κB signaling pathway and the release of proinflammatory cytokines and/or the action of inflammation mediators (HMGB1, iNOS, and NO). These effects are the basis for the antiviral and anticancer activities reported with these pentacyclic compounds or their various glycoside derivatives.
View Article and Find Full Text PDFIn silico analysis of the antidiabetic terpenoid acankoreagenin binding to PPARγ.
In Silico Pharmacol
April 2021
OncoWitan, Wasquehal, 59290 Lille, France.
Acankoreagenin (ACK) is a lupane triterpene found in several and plant species. ACK, also known as acankoreanogenin or HLEDA, bears a major structural analogy with other lupane triterpenoids such as impressic acid (IA) and the largely used phytochemical betulinic acid (BA). These compounds display marked anti-inflammatory, anti-diabetes, and anti-cancer properties.
View Article and Find Full Text PDFImpressic Acid Ameliorates Atopic Dermatitis-Like Skin Lesions by Inhibiting ERK1/2-Mediated Phosphorylation of NF-κB and STAT1.
Int J Mol Sci
February 2021
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea.
Impressic acid (IPA), a lupane-type triterpenoid from , has many pharmacological activities, including the attenuation of vascular endothelium dysfunction, cartilage destruction, and inflammatory diseases, but its influence on atopic dermatitis (AD)-like skin lesions is unknown. Therefore, we investigated the suppressive effect of IPA on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin symptoms in mice and the underlying mechanisms in cells. IPA attenuated the DNCB-induced increase in the serum concentrations of IgE and thymic stromal lymphopoietin (TSLP), and in the mRNA levels of thymus and activation regulated chemokine(TARC), macrophage derived chemokine (MDC), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-13 (IL-13), tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) in mice.
View Article and Find Full Text PDFImpressic Acid Attenuates the Lipopolysaccharide-Induced Inflammatory Response by Activating the AMPK/GSK3β/Nrf2 Axis in RAW264.7 Macrophages.
Int J Mol Sci
January 2021
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea.
Article Synopsis
- Inflammatory diseases result from high levels of inflammation caused by pro-inflammatory mediators and cytokines from macrophages.
- Impressic acid (IPA) was found to reduce LPS-induced inflammation in RAW264.7 cells by decreasing pro-inflammatory cytokines and activating the Nrf2 signaling pathway.
- The study suggests that IPA promotes an anti-inflammatory response through the AMPK/GSK3β/Nrf2 pathway, highlighting its potential in preventing inflammation-related diseases.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!