Objectives: Chronic accumulation of lactate in malignant tumor tissue is associated with increased malignancy and radioresistance. For this study, biopsies of primary head and neck squamous cell carcinoma (HNSCC) and of the normal gingiva of the same patient were compared via metabolic profiling to the healthy gingiva from cancer-free patients.
Materials And Methods: Cryobiopsies of 140 HNSCC patients were used to determine ATP, lactate, and glucose concentrations of the tumor and normal gingiva via induced metabolic bioluminescence imaging (imBI). Additionally, these metabolites were quantified in a collective of 79 healthy (non-tumor-bearing) patients. Furthermore, tumor samples were analyzed via immunofluorescence imaging and quantitative real-time PCR for the expression of lactate and glucose transporters.
Results: There were significant differences in ATP concentrations detectable between the tumor, normal gingiva of tumor patients, and gingiva from healthy patients. Lactate concentrations were significantly increased in tumor tissue compared to the normal gingiva of tumor patients as well as the gingiva from healthy patients. Concerning glucose, there was a significant decrease in glucose concentrations detectable in the tumor biopsies compared to the normal gingiva of tumor patients. On the other hand, tumor samples from patients revealed significantly elevated relative expression levels of monocarboxylate transporters (MCT-1 and MCT-4), as well as glucose transporters (GLUT-1 and GLUT-3) compared to the corresponding normal gingiva of each patient.
Conclusions: We could demonstrate that the lactate concentration in HNSCC correlates with primary tumor (T) stage.
Clinical Relevance: The aim of this study was to identify metabolic parameters to improve early cancer diagnosis, allow predictions on the degree of malignancy, and contribute to a personalized tumor therapy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00784-017-2185-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A study to evaluate the influence of non-axial forces on tooth - a split mouth cross-sectional study.
J Adv Prosthodont
December 2024
Department of Prosthodontics and Crown & Bridge, AB Shetty Memorial Institute of Dental Sciences, NITTE Deemed to be University, Mangalore, India.
Purpose: Proper tooth alignment directs occlusal forces along the long axis, supporting optimal masticatory function and periodontal health. Deviations that lead to non-axial forces are common; however, teeth with such deviations often maintain optimal health. This study aims to assess various occlusal and periodontal parameters in teeth experiencing non-axial forces to better understand the underlying reasons and mechanisms that contribute to their maintained health status.
View Article and Find Full Text PDFOral Manifestations of Vitamin C Deficiency in a Toddler.
J Dent Child (Chic)
September 2024
Department of Pediatric Hematology and Oncology, University of Illinois Chicago, Chicago, Ill., USA.
Vitamin C deficiency, colloquially known as scurvy, has become rare in modern times due to the widespread availability of ascorbic acid-rich foods. Despite this, it continues to be a concern in certain at-risk populations. The purpose of this report is to describe the case of a two-year-old girl who initially presented to a pediatric dental clinic with the chief complaint of hypertrophic gingiva and bleeding.
View Article and Find Full Text PDFManagement of mucogingival defect following excision of multiple gingival squamous cell papillomas using VISTA - A case report with 24 months follow-up.
J Indian Soc Periodontol
December 2024
VISTA Institute for Therapeutic Innovations, California, USA.
Oral squamous cell papilloma is a benign proliferation of the stratified squamous epithelium, associated with the human papillomavirus (HPV). The clinical manifestation includes papillary or verrucous exophytic mass. Complete excision of the lesion, including normal margins, followed by histopathological examination is the treatment of choice.
View Article and Find Full Text PDFInfluence of gingival margin height variation on perceived aesthetics following absent maxillary lateral incisor-canine substitution.
Eur J Orthod
December 2024
Department of Orthodontics, Centre for Craniofacial Development & Regeneration, Faculty of Dentistry, Oral & Craniofacial Sciences, King's College London, London, SE1 9RT, United Kingdom.
Background: Maxillary lateral incisor agenesis is often managed with orthodontic space closure and canine substitution. Anatomic gingival margin heights associated with the maxillary anterior teeth are considered important for achieving aesthetic excellence with space closure, but evidence relating to the perceptions of lay people is poor.
Objectives: This study investigated the influence of gingival margin height variation in the maxillary anterior teeth following orthodontic space closure and canine substitution in the absence of maxillary lateral incisor teeth on perceived aesthetics judged by a lay population.
Merkel cell: Friend or felon.
J Oral Maxillofac Pathol
October 2024
Department of Oral Pathology and Microbiology, ITS-CDSR, Muradnagar, Ghaziabad, Uttar Pradesh, India.
Merkel cells are perceived as tactile receptors within skin and oral mucosa containing abundant intermediate filaments but lacking characteristic condensation of tonofilaments, hence are also referred to as non-keratinocytes. Merkel cell carcinomas (MCCs) are primary aggressive neuroendocrine neoplasms occurring in elderly individuals. Toker in 1972 reported MCC of skin pointing towards sweat glands as the source of origin which was later rectified by Tang with the aid of ultrastructural studies as Merkel cells to be a lineage of such tumours.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!