Aim: To investigate the roles of PKC-α/ezrin signals in phagocytosis crisis of retinal pigment epithelium (RPE) cells in light damage model.
Methods: Light induced mice RPE injury model was established by continuously irradiating cool white light at different exposure time (0, 4, 8h light intensity: 4.18×10 J/cm). , human ARPE-19 cells treated with the doses and intensity (1.57×10 J/cm) of laser irradiation. Histology analysis was evaluated by hematoxylin and eosin (HE) staining. RPE phagocytosis was quantified by measuring the accumulation of photoreceptor outer segments in the sub-retinal space. RPE phagocytosis was assessed by calculating the relative fluorescence intensity of FITC-labeled microspheres in ARPE-19 cells. To further investigate the molecular mechanism, the activation of PKC-α/ezrin signal was evaluated by Western blot and .
Results: HE staining revealed that the thickness of outer nuclear layer decreased significantly after 4 and 8h light exposure. By immunostaining with rhodopsin, a significant greater accumulation of photoreceptor outer segment was noticed after light injury. , light injured RPE cells had less phagocytic activity in a dose dependent manner than that of the normal control (<0.01). Western blot suggested the activation of PKC-α/ezrin signaling was down-regulated in a dose-dependent manner after light exposure.
Conclusion: Our data suggest that light induced phagocytic crisis of RPE cells may result from the down-regulation of PKC-α/ezrin signaling.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5514263 | PMC |
| http://dx.doi.org/10.18240/ijo.2017.07.04 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The co-expression of the depolarizing and hyperpolarizing mechanosensitive ion channels in mammalian retinal neurons.
Front Med (Lausanne)
November 2024
Department of Ophthalmology, Baylor College of Medicine, Houston, TX, United States.
Article Synopsis
- The study investigates the role of mechanosensitive channels (MSCs) in the retina, particularly how they relate to conditions like glaucoma and retinal injuries caused by increased pressure.
- Using advanced techniques, the researchers analyzed the expression of various MSCs in different retinal cells, including Müller cells and retinal ganglion cells (RGCs).
- They found a critical balance between hyperpolarizing and depolarizing MSCs in retinal neurons, suggesting that this balance may affect how vulnerable these neurons are to pressure-induced damage, highlighting potential new avenues for treatment.
Working under Pressure: Scleroderma Presenting with Bilateral Exudative Retinal Detachment in the Context of Scleroderma Renal Crisis: A Case Report.
Case Rep Ophthalmol
October 2024
Department of Ophthalmology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Article Synopsis
- - This case report discusses a unique instance where a 58-year-old female with newly diagnosed systemic sclerosis experienced scleroderma renal crisis, leading to bilateral exudative retinal detachments and severe hypertension.
- - The patient initially presented with vision issues and was found to have malignant hypertension and other symptoms like sclerodactyly, resulting in her diagnosis of diffuse systemic sclerosis and Sjogren's syndrome.
- - After intensive care treatment for her hypertension, the patient's vision and ocular issues improved within three months, highlighting the importance of systemic screening for causes of malignant hypertension, particularly in the presence of systemic sclerosis.
Altered fatty acid distribution in lysosome-associated membrane protein-2 deficient mice.
Biochem Biophys Rep
December 2024
Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
Lysosome-associated membrane protein-2 (LAMP2) deficiency causes the human Danon disease and represents a lysosomal dysfunction because of its pivotal role in regulating autophagy and lysosome biogenesis. LAMP2-deficient mice exhibit a spectrum of phenotypes, including cardioskeletal myopathy, mental retardation, and retinopathy, similar to those observed in patients with Danon disease. Its pathology is thought to involve altered energy metabolism and lipid dysregulation; however, the lipidomic profiles of LAMP2-deficient animals have not been investigated.
View Article and Find Full Text PDFVision Crisis-Bilateral Outer Retinitis Due to Mumps Virus.
Ocul Immunol Inflamm
August 2024
Department of Uveitis and Ocular Immunology Services, Narayana Nethralaya, Bangalore, India.
Purpose: To report a case of mumps-associated outer retinitis, diagnostic, and therapeutic challenges associated with the disease.
Method: Retrospective observational case report.
Results: An 8-year-old male child on presentation had a history of mumps infection following which he developed outer retinitis.
Neovascular Glaucoma: A Rare Presenting Feature of Vogt-Koyanagi-Harada Syndrome.
Cureus
July 2024
Ophthalmology, All India Institute of Medical Sciences, Nagpur, IND.
Article Synopsis
- Vogt-Koyanagi-Harada syndrome (VKH) is a rare autoimmune disorder that causes severe inflammation in multiple systems, primarily affecting the eyes and leading to symptoms like visual loss and skin changes.
- A 20-year-old male patient experienced rapid vision loss due to granulomatous panuveitis and was treated for complications like cataracts and glaucoma, but only later showed signs of vitiligo and poliosis that confirmed VKH.
- This case emphasizes that VKH can present atypically with glaucoma as the first symptom, highlighting the need for early diagnosis to prevent significant vision loss.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!