Differential methylation at in CD4 T cells is associated with multiple sclerosis independently of .

Background: Although many genetic variants have been associated with multiple sclerosis (MS) risk, they do not explain all the disease risk and there remains uncertainty as to how these variants contribute to disease. DNA methylation is an epigenetic mechanism that can influence gene expression and has the potential to mediate the effects of environmental factors on MS. In a previous study, we found a differentially methylation region (DMR) at MHC that was associated within relapsing-remitting MS (RRMS) patients in CD4 T cells. This study aimed to confirm this earlier finding in an independent RRMS cohort of treatment-naïve female patients.

Methods: Total genomic DNA was extracted from CD4 T cells of 28 female RRMS and 22 age-matched healthy controls subjects. DNA was bisulfite-converted and hybridised to Illumina 450K arrays. Beta values for all CpGs were analysed using the DMPFinder function in the MINFI program, and a follow-up prioritisation process was applied to identify the most robust MS-associated DMRs.

Results: This study confirmed our previous findings of a hypomethylated DMR at and a hypermethylated DMR at in this RRMS patient cohort. In addition, we identified a large independent DMR at MHC, whereby 11 CpGs in were hypermethylated in MS cases compared to controls (max. ∆beta = 0.19,  = 2.1 × 10). We did not find evidence that SNP genotype was influencing the DMR in this cohort. A smaller DMR was also identified at , and two non-MHC DMRs at on chr1 and on chr8 were also identified.

Conclusions: The findings from this study confirm our previous results of a DMR at and also suggest hypermethylation in an independent MHC locus, , is associated with MS Taken together, our results highlight the importance of epigenetic factors at the MHC locus in MS independent of treatment, age and sex. Prospective studies are now required to discern whether methylation at MHC is involved in influencing risk of disease onset or whether the disease itself has altered the methylation profile.