AI Article Synopsis
- The study investigates the role of the cAMP-dependent transcriptional regulator Clr in controlling infection events on plant roots through an unknown mechanism.
- Researchers performed transcriptome profiling to identify Clr target genes, discovering that Clr enhances the production of cAMP-dependent succinoglycan and an uncharacterized polysaccharide, while also activating several genes of unknown function.
- The investigation also revealed that genes negatively regulated by Clr are associated with swimming motility and chemotaxis, and two specific Clr-activated genes were essential for managing secondary infections on plant roots.
Article Abstract
The cAMP-dependent transcriptional regulator Clr of regulates the overall number of infection events on roots by a so-far unknown mechanism requiring , a Clr-target gene of unknown function. In order to shed light on the mode of action of Clr on infection and potentially reveal additional biological functions for Clr, we inventoried genomic Clr target genes by transcriptome profiling. We have found that Clr positively controls the synthesis of cAMP-dependent succinoglycan as well as the expression of genes involved in the synthesis of a so-far unknown polysaccharide compound. In addition, Clr activated expression of 24 genes of unknown function in addition to . Genes negatively controlled by Clr were mainly involved in swimming motility and chemotaxis. Functional characterization of two novel Clr-activated genes of unknown function, and , showed that their expression was activated by the same plant signal as . , however, symbiotic expression of proved independent of clr. Both and genes were strictly required for the control of secondary infection on . None of the three and genes were needed for plant signal perception. Altogether this work provides a refined view of the cAMP-dependent Clr regulon of . We specifically discuss the possible roles of genes and other Clr-controlled genes in the control of secondary infection of roots.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498481 | PMC |
| http://dx.doi.org/10.3389/fmicb.2017.01236 | DOI Listing |
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