AI Article Synopsis
- Researchers created genetic constructs with various leader sequences to improve protein expression both inside and outside of cells.
- They developed a unique method to efficiently select clones that produce the highest levels of protein.
- For the intracellular expression using the Pichia pastoris system, the protein yield was measured at 6 mg/liter.
Article Abstract
Genetic constructs with different leader sequences for intra- and extracellular expression of the target protein were generated and an original method for effective selection of clones with maximum expression was developed. For intracellular expression in the Pichia pastoris system, seprin content in cells was 6 mg/liter.
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| http://dx.doi.org/10.1007/s10517-017-3768-0 | DOI Listing |
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