, belonging to Neobatrachia, is a new model organism to study developmental biology, adaptive mechanisms from aquatic to terrestrial life, environmental toxicology, and human disease. use of lungs soon after hatching makes it extremely valuable for the study of lung function and development mechanisms. However, our knowledge of genes and pathways associated with lung development in is very limited. In this study, we conducted transcriptome assembly for the lung of using the Illumina HiSeq4000 platform. We obtained approximately 9.0 GB clean data from the lung of the stage 28 tadpole with lung inflation (NCBI accession numbers: SRP107055). transcriptome assembly identified 209,358 transcripts and 93,813 unigenes. In addition, BLASTX against NR, NT, KO, SwissProt, PFAM, GO and KOG databases were used to annotate all the 93,813 unigenes. This study provides the transcriptome and functional annotation of genes in lung development, which will be useful for comparative transcriptome analyses and promote research into mechanism of lung development in anuran.
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http://dx.doi.org/10.1016/j.gdata.2017.07.002 | DOI Listing |
BMC Med Genomics
January 2025
Department of Surgery, Faculty of General of Medicine, Koya University, Koya, Kurdistan Region - F.R., KOY45, Iraq.
Background: During mammalian spermatogenesis, the cytoskeleton system plays a significant role in morphological changes. Male infertility such as non-obstructive azoospermia (NOA) might be explained by studies of the cytoskeletal system during spermatogenesis.
Methods: The cytoskeleton, scaffold, and actin-binding genes were analyzed by microarray and bioinformatics (771 spermatogenic cellsgenes and 774 Sertoli cell genes).
Sci Data
January 2025
Marine Biotechnology Fish Nutrition and Health Division, Central Marine Fisheries Research Institute, Post Box No 1603 Ernakulam North PO., Kochi, 682018, Kerala, India.
Mussels, particularly Perna viridis, are vital sentinel species for toxicology and biomonitoring in environmental health. This species plays a crucial role in aquaculture and significantly impacts the fisheries sector. Despite the ecological and economic importance of this species, its omics resources are still scarce.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, PR China; Frontier Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin 300072, PR China. Electronic address:
In this study, we successfully integrated the full-length genome of the cyanophage PP into the non-host cyanobacterium Synechococcus elongatus PCC 7942, facilitated by conjugation via Escherichia coli. To address the challenge posed by the toxic open reading frames (ORFs) of PP in E. coli, we first identified and characterized three toxic ORFs.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA.
We analyzed the transcriptome data of wildtype and estrogen receptor β knockout () rat ovaries during the early postnatal period and detected remarkable changes in epigenetic regulators and transcription factors. Compared with postnatal day (PD) 4.5 ovaries, PD 6.
View Article and Find Full Text PDFMar Genomics
March 2025
Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou, 350108, China.
This is the first report of a transcriptome assembly of a newly discovered a new Protocruzia species sampled from the under-sampled area near the Mariana Trench. We sequenced the transcriptome of P. marianaensis using the Illumina Novaseq 6000 platform.
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