Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spike-in oligonucleotides (sRNA spike-ins) that enable absolute normalization of sRNA-Seq data across independent experiments, as well as the genome-wide estimation of sRNA:mRNA stoichiometries when used together with mRNA spike-in oligonucleotides.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5517642 | PMC |
| http://dx.doi.org/10.1038/s41598-017-06174-3 | DOI Listing |
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