Carbapenem-resistant Gram-negative bacteria (CR-GNB) have emerged and disseminated worldwide, become a great concern worldwide including Korea. The prevalence of fecal carriage of imipenem-resistant Gram-negative bacteria (IR-GNB) in persons in Korea was investigated. Stool samples were collected from 300 persons upon medical examination. Samples were screened for IR-GNB by using MacConkey agar with 2 μl/ml imipenem. Species were identified by 16S rRNA gene sequence analysis, and antimicrobial susceptibility was determined by the broth microdilution method. In total, 82 IR-GNB bacterial isolates were obtained from 79 (26.3%) out of 300 healthy persons. Multilocus sequence typing analysis showed very high diversity among IR P. aeruginosa, S. maltophilia, and E. cloacae isolates, and pulsed-field gel electrophoresis revealed five main pulsotypes of IR P. mirabilis. As for the presence of metallo-β-lactamases (MBLs), only one IMP-25-producing S. marcescens isolate was identified. Although only one carbapenemase-producing isolate was identified, the high colonization rates with IR-GNB isolates in this study is notable because carriers may be a reservoir for the dissemination of resistant pathogens within the community as well as in health care institutions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12275-017-6555-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Re-sensitization of imipenem-resistant Pseudomonas aeruginosa and restoration of cephalosporins susceptibility in Enterobacteriaceae by recombinant Esterase B.
Lett Appl Microbiol
December 2024
Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand.
Sphingobium sp. SM42 Esterase B (EstB) is an enzyme with a dual function in degrading dibutyl phthalate and catalyzing the cleavage of the C-S bond in C3-sidechains of the dihydrothiazine ring of cephalosporins, generating more active β-lactam derivatives. Global prokaryotic genome analysis revealed the existence of a gene identical to estB in Pseudomonas aeruginosa strain PS1 suggesting a horizontal gene transfer event involving estB.
View Article and Find Full Text PDFEmergence of resistance to last-resort antimicrobials in bacteremia patients: A multicenter analysis of bloodstream pathogens in Korea.
PLoS One
October 2024
Department of Laboratory Medicine, Sanggye Paik Hospital, School of Medicine, Inje University, Seoul, South Korea.
Article Synopsis
- * Out of 3,397 bacterial isolates from 3,094 patients, 949 were classified as multidrug-resistant organisms (MDRO), highlighting significant resistance to commonly used antibiotics, including imipenem, methicillin, and vancomycin.
- * Although the antibiotic spectrum coverage (ASC) scores were similar for MDRO and non-MDRO groups initially, the MDRO group showed significant improvements in coverage two days later, indicating a need for faster diagnostic methods to combat emerging resistance.
RESIST ACINETO test for the rapid detection of NDM and OXA acquired carbapenemases directly from blood culture in species.
Microbiol Spectr
October 2024
UMR Processus Infectieux en Milieu Insulaire Tropical, CNRS 9192, INSERM U1187, IRD 249, Université de La Réunion, Saint-Denis, La Réunion, France.
Article Synopsis
- Carbapenem-resistant Acinetobacter baumannii (CRAB) is a major global health concern, particularly in bloodstream infections, making early detection vital for effective treatment.
- The RESIST ACINETO immunochromatographic assay accurately detects key carbapenemases (OXA-23, OXA-40/58, NDM) in clinical isolates, demonstrating perfect sensitivity and specificity in both bacterial and blood cultures.
- Utilizing RESIST ACINETO can enhance rapid identification of CRAB in high-incidence regions like the Balkans and Southeast Asia, facilitating optimized treatment strategies, especially with alternatives like cefiderocol.
activity of cefiderocol and comparator antibiotics against multidrug-resistant non-fermenting Gram-negative bacilli.
JAC Antimicrob Resist
February 2024
Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Background: This study evaluated the activity of cefiderocol, ceftazidime/avibactam, and aztreonam/avibactam against clinically important multidrug-resistant non-fermenting Gram-negative bacilli.
Methods: Bacteraemic isolates of 126 multidrug-resistant (MDRAB), 110 imipenem-resistant [including 14 difficult-to-treat resistant (DTRPA)], 45 beta-lactam-non-susceptible complex (BCC), 47 levofloxacin or trimethoprim/sulfamethoxazole-non-susceptible and 22 ciprofloxacin-non-susceptible spp. collected between 2019 and 2021 were subjected to MIC determination for cefiderocol, ceftazidime/avibactam and aztreonam/avibactam.
Detection of Carbapenemase Encoding Gene and Resistance to Cefiderocol in Hospital and Community eXtensive Drug Resistance and Carbapenem-Resistant Strains in Morocco.
Foodborne Pathog Dis
October 2023
Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco.
Article Synopsis
- A study in Morocco analyzed 134 Pseudomonas aeruginosa (Pa) isolates, revealing that 18 were resistant to carbapenems, critical antibiotics for treating severe infections.
- Advanced testing methods confirmed the resistance and identified specific genes responsible for this problem, highlighting a worrying trend of multidrug resistance among these pathogens.
- The findings underscore the urgent need for careful antibiotic selection and monitoring, as most isolates exhibited extensive drug resistance, though some remained susceptible to colistin and cefiderocol.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!