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Screening of specific nucleic acid aptamers binding tumor markers in the serum of the lung cancer patients and identification of their activities. | LitMetric

AI Article Synopsis

  • Lung cancer is the leading cause of cancer deaths globally, but early detection remains a challenge, necessitating new diagnostic methods.
  • This study focuses on developing specific nucleic acid aptamers that can bind to tumor markers in the serum of lung cancer patients, utilizing advanced screening techniques like SELEX and real-time PCR.
  • After extensive screening, six aptamers were identified, with aptamer 43 showing the highest specificity for target molecules, offering potential breakthroughs for early lung cancer diagnosis and treatment.*

Article Abstract

Lung cancer is by far the leading cause of cancer death in the world. Despite the improvements in diagnostic methods, the status of early detection was not achieved. So, a new diagnostic method is needed. The aim of this study is to obtain the highly specific nucleic acid aptamers with strong affinity to tumor markers in the serum of the lung cancer patients for targeting the serum. Aptamers specifically binding to tumor markers in the serum of the lung cancer patients were screened from the random single-stranded DNA library with agarose beads as supports and the serum as a target by target-substituting subtractive SELEX technique and real-time quantitative polymerase chain reaction technique. Subsequently, the secondary single-stranded DNA library obtained by 10 rounds of screening was amplified to double-stranded DNA, followed by high-throughput genome sequence analysis to screen aptamers with specific affinity to tumor markers in the serum of the lung cancer patients. Finally, six aptamers obtained by 10 rounds of screening were identified with high specific affinity to tumor markers in the serum of the lung cancer patients. Compared with other five aptamers, the aptamer 43 was identified both with the highest specificity to bind target molecule and without any obvious affinity to non-specific proteins. The screened aptamers have relatively high specificity to combine tumor markers in the serum of the lung cancer patients, which provides breakthrough points for early diagnosis and treatment of lung cancer.

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Source
http://dx.doi.org/10.1177/1010428317717123DOI Listing

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