Puzihella rosea gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from freshwater.

Two Gram-stain-positive, aerobic, pink, curved, rod-shaped, non-motile bacterial strains, designated MI-28T and SKY-11, were isolated from freshwater samples taken from a river and fish pond, respectively. Based on characterization using a polyphasic approach, the two strains showed highly similar phenotypic, physiological and genetic profiles. They demonstrated 99.9 % 16S rRNA gene sequence similarity and a 93-95 % DNA-DNA relatedness value, suggesting that they represent a single genomic species. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strains MI-28T and SKY-11 form a distinct lineage with respect to closely related genera within the family Microbacteriaceae of the class Actinobacteria, which is most closely related to Rhodoluna and Pontimonas, and levels of 16S rRNA gene sequence similarity with the type species of related genera were less than 95 %. Cell-wall analysis showed that the peptidoglycan contained 2,4-diaminobutyric acid, alanine, glycine and glutamic acid. The predominant fatty acids were iso-C14 : 0, anteiso-C15 : 0 and iso-C16 : 0. The polar lipid profile consisted of a mixture of phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized glycolipid and an uncharacterized aminophospholipid. The major polyamine was putrescine. The major isoprenoid quinone was MK-10. The G+C content of DNA was between 62.6 and 62.9 mol%. On the basis of the genotypic and phenotypic data, strains MI-28T and SKY-11 represent a novel genus and species of the family Microbacteriaceae, for which the name Puzihella rosea gen. nov., sp. nov. is proposed. The type strain of the type species is MI-28T (=BCRC 80688T=LMG 27848T=KCTC 29239T).