AI Article Synopsis
- The study introduces a flexible lactate biosensor utilizing l-lactate dehydrogenase and NAD+ immobilized on a nickel oxide film, enhanced by graphene oxide and magnetic beads for better performance.
- The biosensor shows impressive sensitivity of 45.397 mV/mM and a high linearity of 0.992 within the lactate concentration range of 0.2 mM to 3 mM.
- Electrochemical impedance spectroscopy results indicate that the modified biofilm has superior electron transfer efficiency compared to other configurations, and additional tests confirm its detection limits and resilience.
Article Abstract
We proposed the flexible arrayed lactate biosensor based on immobilizing l-lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide ( NAD + ) on nickel oxide (NiO) film, and which the average sensitivity could be enhanced by using graphene oxide (GO) and magnetic beads (MBs). By using GO and MBs, it exhibits excellent sensitivity (45.397 mV/mM) with a linearity of 0.992 in a range of 0.2 mM to 3 mM. According to the results of electrochemical impedance spectroscopy (EIS), the electron transfer resistance of LDH- NAD + -MBs/GPTS/GO/NiO film was smaller than those of LDH-NAD⁺/GPTS/GO/NiO film and LDH- NAD + /GPTS/NiO film, and it presented the outstanding electron transfer ability. After that, the limit of detection, anti-interference effect and bending test were also investigated.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539599 | PMC |
| http://dx.doi.org/10.3390/s17071618 | DOI Listing |
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